拟南芥温敏雄性不育突变体atms1的获得及表型分析  

Thermo-Sensitive Male Sterile Mutant atms1 of Arabidopsis thaliana Access and Phenotypic Analysis

作　　者：戴文懿[1] 孙亚梅[1] 高贝[1] 周树敏[1] 袁晓君[1] 韦嘉励[1] 张卫[1] 

机构地区：[1]上海大学生命科学学院,上海200444

出　　处：《上海大学学报（自然科学版）》2011年第5期681-686,共6页Journal of Shanghai University:Natural Science Edition

基　　金：上海市科委浦江人才计划资助项目(08PJ1405500);上海市优秀青年教师科研专项基金资助项目

摘　　要：从甲基磺酸乙酯(ethylmethane sulfonate,EMS)化学诱变建立的野生型拟南芥(Col-0)突变体文库中筛选到1株突变体.在低温16℃时,该突变体的雄性育性与野生型没有显著差异,花粉染色呈现100%可育.随着培养环境温度的升高,突变体花粉育性逐渐下降,因此,该突变体为一温敏雄性不育突变体,并被命名为atms1(ambienttemperature-sensory male sterility 1,即环境温度敏感雄性不育1).花药切片结果显示,在23℃以下,该突变体花药各个发育时期的形态与野生型花药没有显著的差异;而27℃处理1周后的突变体花药呈现多种表型:同一朵花中各个花药的发育时期出现显著分化,花粉母细胞胼胝体单薄,绒毡层发育滞后于同时期的野生型花药.遗传分析确定,atms1的不育表型是由单个隐性核基因控制的.An ambient temperature Arabidopsis thaliana male sterile mutant ambient temperature-sensory male sterile 1（atms1）,generated from ethylmethane sulfonate（EMS） mutagenesis has been identified.At low temperature 16 ℃,atms1 showed 100% pollen viability and silique fertility,while with the culture temperature increased,atms1 showed decreasing pollen viability and silique fertility.Therefore atms1 is a temperature sensory male sterile mutant.The morphological assay demonstrated that atms1 anther showed few significant impairments compared with wild-type anther when ambient temperature was below 23 ℃.At higher temperature 27 ℃,the atms1 anther exhibited pleiotropic phenotypes： the developmental phases of anthers within one flower differed significantly;the callose layer of pollen mother cell of mutant was much thinner than that of wild-type,and the atms1 tapetum development exhibited much retarded than corresponding wild-type tapetum.Genetic assay demonstrated that the phenotypes of atms1 were controlled by a single recessive locus.

关 键 词：拟南芥 温敏雄性不育 花粉 花药 绒毡层 

分 类 号：Q94[生物学—植物学]