辣椒叶片基因组DNA提取方法的研究  

The Extraction of Genomic DNA from Hot Pepper

作　　者：周春阳[1] 谢立波 李景富[1] 周宇 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030 [2]黑龙江省农科院园艺分院,哈尔滨150069

出　　处：《辣椒杂志》2011年第3期35-37,共3页Journal of China Capsicum

基　　金：航天育种工程项目(2009HT2000);黑龙江省自然基金重点项目(ZD200818-01);黑龙江省自然基金项目(C200943);国家大宗蔬菜产业技术体系哈尔滨综合试验站项目(CARS-25-G-11)

摘　　要：本项研究采用SDS法、CTAB1法、CTAB2法及CTAB3法对辣椒叶片基因组DNA进行提取,通过琼脂糖凝胶电泳法与紫外吸收检测法对DNA的纯度进行检测。试验证明几种提取方法均能提取出辣椒叶片的基因组DNA,其中CTAB3法提取的DNA经琼脂糖凝胶电泳检测得到一条迁移率很低的整齐清晰的DNA条带,所提取DNA的质量和纯度较高。紫外吸收检测结果表明,CTAB3法提取的辣椒叶片DNA具有典型天然DNA分子的标准紫外吸收光谱特点。所以此方法为辣椒叶片基因组DNA提取的最佳方法。In this experiment, SDS,CTAB 1 ,CTAB 2 and CTAB 3 methods were used to extract DNA from hot pepper leaves, and agarose gel electrophoresis and UV-spectrophotometry were done to test DNA purity. The results indicated that all the methods could extract DNA from hot pepper leaves; the DNA extracted with the CTAB 3 method showed a consistent and clear DNA band of very low migration rate in the agarose gel electrophoresis, had higher quality and purity, and got the typical absorption spectra in the UV-spectrophotometry. So, it is the best method in this study.

关 键 词：辣椒 DNA提取 琼脂糖凝胶电泳检测 紫外吸收检测 

分 类 号：S641.3[农业科学—蔬菜学]