鹅掌楸属树种繁殖性能的遗传分析  

Genetic Dissection for the Reproductive Fitness of Liriodendron Derived from Offsprings of Complete-Diallel Crosses

作　　者：冯源恒[1,2] 李火根[1] 王龙强[1] 边黎明[1] 姚俊修[1] 施季森[1] 

机构地区：[1]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京210037 [2]广西壮族自治区林业科学研究院,南宁530001

出　　处：《林业科学》2011年第9期43-49,共7页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30972391;31170621);国家"十一五"科技支撑课题(2006BAD01A16);江苏省高校自然科学重大项目(10KJA220017)

摘　　要：分析影响鹅掌楸繁殖性能的遗传效应。以鹅掌楸、北美鹅掌楸各4株成年个体作为交配亲本,进行8×8全双列交配设计,以各交配组合子代的出苗率来评价各亲本的雌、雄繁殖性能,分析影响鹅掌楸繁殖性能的父本效应、母本效应、父母本交互作用效应、各亲本的一般配合力及各交配组合的特殊配合力。同时,利用11个SSR位点信息估算亲本间遗传距离,分析亲本间遗传距离与繁殖性能之间的相关性。结果表明:鹅掌楸繁殖性能主要受母本效应影响,父本效应可以忽略;繁殖性能的一般配合力亲本间差异较大,且雌性大于雄性;种内交配的特殊配合力远高于种间杂交。尽管亲本遗传距离与杂交亲和性二者间相关不显著,但仍存在如下趋势:种内杂交的亲和性随遗传距离增大而增加;而种间杂交的亲和性则随遗传距离增大而降低。研究结果对于鹅掌楸杂交种子园的亲本选配及杂种优势利用有重要的参考意义。In this paper,the genetic effects on the reproductive fitness of Liriodendron were analyzed based on a 8×8 complete-diallel crossing,in which 4 adult L.chinense and 4 adult L.tulipifera were randomly chosen as mating parents.The seed germination ratio for each mating combination was used as an index to evaluate male/female reproductive fitness for its corresponding mating parents.The genetic effects on reproductive fitness,in terms of maternal effect,paternal effect,their interaction effect,general combining ability and specific combining ability,were further dissected.Meanwhile,in order to explore the relationship between the genetic distances（GD） of mating parents and their cross-compatibilities,11 SSR loci were amplified in 8 mating parents to estimate GD for each parent pair.The results are as follows：the reproductive fitness of Liriodendron was mostly dominated by mother tree,whereas the paternal effect could be ignored.There existed a reasonable difference in the combining ability of reproductive fitness among 8 parents,with more for female reproduction than for male reproduction.The special combining ability of reproductive fitness in intraspecies mating was much bigger than that of interspecific crossing.Although the relationship between parent pair＇s GD and their cross-compatibility was not significant,there was a trend that the cross-compatibility increased as parent pair＇s GD augmented in intraspecific mating;while a reverse trend appeared in interspecific mating.These results above would provide valuable knowledge for parent pair selection in hybrid seed orchard and the utilization of heterosis in Liriodendron.

关 键 词：鹅掌楸 繁殖性能 遗传效应 遗传距离 杂交亲和性 

分 类 号：S718.46[农业科学—林学] S722.34