贵州省羊口疮病例分子生物学诊断  

Molecular Diagnosis of the ORF Cases in Guizhou Province

作　　者：刘廷江[1] 程振涛[1,2] 向智龙[1,2] 江楠[1,2] 龙雨清[1] 李廷强[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病研究室,贵州贵阳550025

出　　处：《贵州畜牧兽医》2011年第5期8-11,共4页Guizhou Journal of Animal Husbandry & Veterinary Medicine

基　　金：贵州大学大学生SRT项目"羊传染性脓疱病毒贵州株的PCR鉴定及分子特征分析"(贵大SRT(2010)021号);贵州大学引进人才科研项目"贵州省羊口疮的诊断与防控技术研究"(贵大人基合字(2009)024号);贵州省科学技术基金项目"贵州省羊口疮的病原学与检测技术研究"(黔科合J字[2010]2260)

摘　　要：为了更好地鉴别诊断疑似羊口疮病例,采用PCR技术、分子克隆技术和生物信息技术对毕节地区羊口疮疑似病例进行实验室诊断。结果显示,从疑似病例组织病料中扩增出与预期大小相符的1 137 bp的B2L基因片断,且该基因核苷酸同源性与GenBank中已发表羊口疮病毒序列间的同源性为97.1%～99.1%。试验结果表明,该病例为羊口疮病毒感染。研究结果为羊口疮临床病例的快速鉴别诊断提供了1种快速可靠的方法。In order to differential diagnosis of suspected off （ sheep contagious pustular dermatitis） cases, PCR, molecular cloning and biological information technology have been used to d/agnosis the off suspected cases of Bijie city. The results showed that suspected cases was amplified w/th the expected size of 1 137 bp of the B2L gene which nucleotide homology with ttae GenBank published off virus sequence homology was 97.1% to 99.1%. The results certified that the suspected cases were caused by off virus infection and provided a fast and reliable method for the rapid differential diagnosis of orf clinical eases.

关 键 词：羊口疮 分子生物学 诊断 

分 类 号：S858.26[农业科学—临床兽医学]