清开灵注射液中黄芩苷的药代动力学研究  

Pharmacokinetic Study on baicalin in Qingkailing Injection

作　　者：杨萍[1] 谢云[1] 

机构地区：[1]湖北中医药大学药学院

出　　处：《湖北中医药大学学报》2011年第5期28-30,共3页Journal of Hubei University of Chinese Medicine

摘　　要：目的建立清开灵注射液中黄芩苷血药浓度分析方法并研究其在家兔体内药物动力学过程,获得相关药动学参数。方法采用Venusil ASB C18色谱柱(250 mm×4.6mm,5μm);流动相:乙腈-水-磷酸(23∶77∶0.18);流速:1 mL/min;检测波长:276 nm;柱温:30℃。结果清开灵注射后其黄芩苷成分在家兔体内呈二室模型分布,经DAS2.1.1程序处理,得到主要药动学参数:血药浓度时间曲线下面积(AUC0-t)为427.792(mg/L)×min,AUC0-∞为497.026(mg/L)×min,最大血药浓度(Cmax)为22.3mg/L,清除率(CLz)为0.091(L/min)/kg,半衰期(t1/2z)为83.345min,表观分布容积(VZ)为10.889L/kg,达峰时间(Tmax)为5min。结论本法简便、准确、可靠,可用于测定血清中黄芩苷的含量。Objective To establish a method for determination the blood concentration of baicalin and study the pharmac-okinetics of baicalin in rabbits to obtain pharmacokinetic parameters.Methods The Venusil ASB C18 column（250 mm×4.6 mm,5μm）was used and the HPLC conditions were acetonitrile：water：phosphoric acid（23∶77∶0.18）.The flow rate was 1 mL/min.The detection wave length was 276 nm and 30 ℃ column temperature. Results The blood concentration-time data of baicalin in rabbits was found to be in accordance with the 2-compartment model.The DAS2.1.1 program was used to process pharmacokinetic parameters and its pharmacokinetics parameters were： AUC（0-t） = 427.792 mg/L·min,AUC（0-∞） = 497.026 mg/L·min,Cmax=22.3 mg/L,CLz=0.091L/min/kg,t1/2z=83.345min,Vz=10.889L/kg,Tmax=5min.Conclusion The method is simple,accurate and reliable,therefore could be used to determine the concentration of baicalin in serum.

关 键 词：清开灵注射液 黄芩苷 药物动力学 高效液相色谱法 

分 类 号：R285.5[医药卫生—中药学]