RT-PCR法克隆广谱抗病基因TGA2及其蛋白表达载体构建  

Amplified by RT-PCR and protein expressing vector construction of broad-spectrum disease-resistance TGA2 gene

作　　者：刘永光[1] 孙向阳[1] 刘克锋[2] 

机构地区：[1]北京林业大学水土保持学院,北京100083 [2]北京农学院城乡发展学院,北京102206

出　　处：《河北农业大学学报》2011年第5期47-52,共6页Journal of Hebei Agricultural University

基　　金：北京市教委面上项目资助(KM200910020014);北京市园林绿化局治沙办项目资助(2008)

摘　　要：本试验采用Trizol Reagent提取拟南芥总RNA,设计相关引物,采用反转录PCR方法克隆TGA2转录因子;利用酶切连接方法,将该基因正向导入蛋白表达载体。经过相关检验,TGA2转录因子连接到pMXB10载体内含肽(Intein)的N端,成功构建包含TGA2转录因子的pMXB10-TGA2蛋白表达载体。After extracting the Arabidopsis thaliana RNA with trizol reagent and designing primes,TGA2 transcription factor gene was cloned by RT-PCR.The experiment connects the TGA2 transcription factor with intein＇s N-Terminal of pMXB10 vectors.The TGA2 transcription factor gene was correctly cloned into protein expression vectors.The experiment achieved the pMXB10-TGA2 protein expression vectors containing TGA2 transcription factor.

关 键 词：TGA2转录因子 SAR 广谱抗病 载体构建 

分 类 号：Q789[生物学—分子生物学]