美洲大蠊Per a 9的抗原表位特征及三维结构建模  

Characterization of antigenic epitopes of Per a 9 from Periplaneta americana and its three dimensional structure modeling

作　　者：胡玉静[1] 金珊珊[1] 杨海伟[1] 魏继福[1] 何韶衡[1] 

机构地区：[1]南京医科大学第一附属医院临床实验研究中心,南京210029

出　　处：《生物学杂志》2011年第5期1-5,共5页Journal of Biology

基　　金：国家自然科学基金(30801016;30972822;81001329;81030054)

摘　　要：Per a 9是美洲大蠊主要过敏原蛋白之一。通过生物信息学方法了解美洲大蠊过敏原蛋白Per a 9的结构特征,为蟑螂变态反应性疾病的诊断和治疗提供线索。BLAST得到Per a 9相似序列,构建同源进化树,结果显示美洲大蠊Per a 9与德国小蠊精氨酸激酶在进化上具有较近的亲缘关系。Per a 9主要为α+β结构的亲水性蛋白,其主要抗原表位集中于33—46、55—74、89—117、123—135、199—217、235—243、251—266、286—354区域。Motif预测其具有1个鸟嘌呤磷酸转移酶活性位点,6个蛋白激酶C磷酸化位点,7个酪氨酸激酶II磷酸化位点和2个N豆蔻酰化位点。其预测的三维结构基本能反应Per a 9真实的空间构象,这将为今后进一步了解和掌握Per a 9结构和功能的关系打下理论基础。Per a 9 was one of the major allergens of Periplaneta americana.The bioinformatic analysis was used to identify and characterize the structural properties of Per a 9 for the diagnosis and therapy of cockroach allergy.BLAST was applied to search for the high similar protein to Per a 9.Clustal W 1.83 was used for sequence alignment and phylogenetic tree construction.The phylogenetic analysis found that Per a 9 showed highest similarity with the arginine kinase identified from Blattella germanica.Per a 9 was a ubiquitous protein,mainly containing α＋β secondary structure.Its antigenic epitope was mostly located on 33—46、55—74、89—117、123—135、199—217、235—243、251—266 and 286—354 regions.Motif analysis found that Per a 9 had a guanido phosphotransferases active site,six protein kinase C phosphorylation sites,seven casein kinase Ⅱphosphory-lation sites and two N-myristoylation sites.Three-dimensional structure of Per a 9 was also constructed.It seemed that the predicted structure could reflect the real tertiary structure of Per a 9 and was useful for the further study of structure-function relationship.

关 键 词：美洲大蠊 PER A9 抗原表位 三维结构建模 

分 类 号：Q819[生物学—生物工程]