粪产碱杆菌来源的青霉素G酰化酶拆分制备(S)-2-氨基-4-苯基丁氨酸  

Preparing(S)-2-Amino-4-phenylbutyric Acid by Enantioselective Hydrolysis of N-Phenylacetyl-2-amino-4-phenylbutyric with Penicillin G Acylase from Alcaligenes faecalis

作　　者：许伟钦[1] 林金萍[1] 魏东芝[1] 

机构地区：[1]华东理工大学鲁华生物技术研究所,上海200237

出　　处：《华东理工大学学报（自然科学版）》2011年第5期538-543,共6页Journal of East China University of Science and Technology

摘　　要：粪产碱杆菌来源的青霉素G酰化酶(A lcaligenes faecalispenicillin G acylase,A-fPGA)可以对映选择性地水解N-苯乙酰苯基丁氨酸制备(S)-2-氨基-4-苯基丁氨酸。反应体系的pH及温度对酶的对映选择性影响不显著。研究表明,水饱和的乙酸乙酯体系要优于传统的水相体系,该体系显著提高了底物N-苯乙酰化苯基丁氨酸的溶解度,并可简化产物的分离过程。当底物的转化率为48.6%时,(S)-2-氨基-4-苯基丁氨酸的光学纯度(e.e.值)为99.8%。（S）-2-amino-4-phenylbutyric acid can be prepared by enantioselective hydrolysis of N-phenylacetyl-2-amino-4-phenylbutyric with penicillin G acylase from Alcaligenes faecalis（AfPGA）.pH vaule and temperature during the hydrolysis process had no significant influence on enantioselectivity of AfPGA.Compared with water phase,acetic ester with saturated content of water was an ideal reaction system for aforementioned hydrolysis process.Significant promotion in solubility of substrates was observed with acetic ester phase.It was also more convenient to recover the products from acetic ester with saturated content of water.When the substrate conversion rate was 48.6%,the e.e.value of（S）-2-amino-4-phenylbutyric acid achieved 99.8%.

关 键 词：青霉素G酰化酶 (S)-2-氨基-4-苯基丁氨酸 光学选择性 

分 类 号：Q814[生物学—生物工程] Q819