地肤子活性成分对山楂叶螨酯酶同工酶的影响  

Effects of the Chloroform Extracts of Kochia Scoparia(L.) Schrad on Several Enzyme Systems in Tetranychus Viennensis

作　　者：张慧荣[1] 刘素琪[2] 王海香[3] 郝晓娟[2] 莫海波[2] 曹挥[2] 

机构地区：[1]太原市园林局,山西太原030001 [2]山西农业大学农学院,山西太谷030801 [3]山西农业大学林学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2011年第4期321-325,共5页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：山西省青年基金(2010021031-1);山西农业大学科技创新基金(2009019;2009013)

摘　　要：实验研究了地肤子活性成分KFCE-2处理后,山楂叶螨体内的酯酶同工酶的影响。从聚丙烯酰胺凝胶电泳结果来看,在处理组中,没有检测到新的其它酶带。并且其酯酶酶带E1、E2比对照组的颜色要浅,而且图谱面积小;而E3在处理的12h后完全消失。酯酶同工酶电泳图谱经UVP2凝胶成像系统扫描分析,从强度变化上可以看出,E2、E3带的活性从处理的4h(LANE-a')开始活性就在逐渐减弱,尤其是E3带的活性在处理的12h后(LANE-c')就完全被抑制。此外,在迁移率的变化上,处理组E1、E2带的迁移率有逐渐增大的趋势;E3带的迁移率在能观察到的两个时间内,有减小的趋势。这表明KFCE-2可能通过抑制山楂叶螨体内的酯酶同工酶的活性,并且通过特异性的结合,改变部分酯酶的活性结构,来降低其解毒代谢。这也表明了地肤子作为新型植物源农药具有良好的开发前景。The changes of the activities of the esterase isoenzyme in Tetranychus Viennensis treated with chloroform extract of Kochia Scoparia（KFCE-2） were tested.The results of the polyacrylamide gel electrophoresis（PAGE） indicated that new band was not found in mites treated with KFCE-2,and the colors of the esterase bands E1 and E2 were lighter than that of the control group and the area of the maps were smaller.The band of E3 disappeared completely when treated after 12 h.When the electropherograms of PAGE were analyzed used UVP2 gel imaging system,we found from the change of intensity that the activities of the E2 and E3 bands tended to be weak after treated 4 h（LANE-a＇）,especially the activity of the band E3 which was entirely suppressed after 12 h（LANE-c＇）.Besides,the migration rate of the E1 and E2 bands tended to be enlarged and that of the E3 band was gradually decreased in the treated groups.The result showed that KFCE-2 could reduce the antidote metabolism of Tetranychus Viennensis by restraining the activities of esterase isoenzyme and by changing the partly active structure of esterase through the specific combination which means that there has good prospect of development of the Kochia Scoparia as a natural pesticide.

关 键 词：地肤 山楂叶螨 酯酶同工酶 

分 类 号：S436.619[农业科学—农业昆虫与害虫防治]