生长抑素对家兔膝关节骨性关节炎自由基代谢的影响  

Effect of somatostain intra-articular injection on metabolism of free radicals in rabbits with knee osteoarthritis

作　　者：张振涛[1] 王一璇[2] 刘兆华[3] 

机构地区：[1]廊坊市人民医院骨科,廊坊065000 [2]中国石油中心医院康复科,廊坊065000 [3]廊坊卫生职业学院,廊坊065000

出　　处：《国际外科学杂志》2011年第10期684-687,共4页International Journal of Surgery

摘　　要：目的探讨生长抑素对家兔膝关节骨性关节炎动物模型自由基代谢的影响，从而探讨生长抑素关节腔内注射治疗骨性关节炎的可能作用机制。方法取Et本大耳白兔40只，随机分成正常对照组、空白模型组、0．9％的氯化钠溶液组、生长抑素组。造模后向生长抑素组右侧膝关节腔内注射生长抑素溶液；向0．9％的氯化钠溶液组右侧膝关节腔内注射0．9％的氯化钠溶液。在造模后10周测量家兔右后膝关节活动度，测定血清一氧化氮和透明质酸的含量，测定滑膜组织中超氧化物歧化酶活性及丙二醛含量。结果生长抑素组治疗后的兔膝关节活动度为（96．01±1．06）度，较治疗前（50．21±1．80）度明显改善，空白模型治疗后的兔膝关节活动度为（51．89±1．89）度，治疗前为（50．76±1．44）度，改善不明显，两组间差异具有统计学意义（P=0．0025，P〈0．01）；生长抑素组血清一氧化氮含量为（80．14±1．67）μmol／mL，HA含量为（133．73±2．75）μmol／mL，空白模型组血清一氧化氮含量为（111．60±2．76）μmol／mL，HA含量为（309．11±1．89）μmol／mL，两组组间差异有显著统计学意义（P1=0．0049，P2=0．0052，P〈0．01）；生长抑素组滑膜组织超氧化物歧化酶活性（24．74±1．67），与空白模型组（15．77±2．76）相比明显升高，差异有显著统计学意义（P=0．0046，P〈0．01），丙二醛含量（0．89±1．46），与空白模型组（1．33±1．03）相比明显降低，且差异有显著统计学意义（P=0．0039，P〈0．01）。结论关节腔内注射生长抑素可以改善家兔膝关节骨性关节炎的氧化应激损伤，提高膝关节的活动度。Objective To discuss the effect of somatostain SS on metabolism of free radicals in animal models of osteoarthritis, so to discuss the treatment mechanism of intra-articular injection of somatostatin on osteoarthritis. Methods Forty experimental Japanese big-ear rabbits were randomly divided into four groups ： normal control group, blank model group, normal saline group and SS group, n = 10 for each group. After modeling, the rats in SS group and normal saline group received intra-articular injection of somatostain and saline respectively on the right knee. Ten weeks after the modeling, we measured the movement of right knee joint in rabbits, the levels of nitric oxide （ NO ） and hyaluronic acid （ HA ） in serum from the heart blood, and the activity of SOD and the content of MDA in synovial tissue. Results Compared with the blank model group, the movement of the knee joint was significantly increased in SS group, （96.01 ± 1.06 ） vs （50.21 ± 1.80） （P 〈 0.01 ）. In the blank model group, the levels of NO and HA （μmol/mL）in serum were （ 111.60 ± 2.76） and （ 309.11 ± 1.89 ） , which was significantly decreased in SS group, which was （ 80. 14 ± 1.67） and （ 133.73 ± 2.75 ） （ P1 = 0. 0049, P2 = 0. 0052, P 〈 0.01 ）. Compared with the blank model group, the SOD activity was （ 15.77 ~ 2.76 ） and the MDA in serum nmol/mg prot was （ 1.33 ± 1.03） , while was significantly increased in SS group, （24.74 ± 1.67）（P 〈0.01 ）, and the levels of MDA in serum was significantly decreased in the SS group, （0.89 ± 1.46 ） （ P 〈 0.01 ）. Conclusion Intra- articular injection of somatostatin in knee osteoarthritis can improve the oxidative stress, enhance the activity of the knee joint.

关 键 词：生长抑素 膝骨性关节炎 自由基代谢 

分 类 号：R965[医药卫生—药理学]