桑生除痹合剂对兔膝骨性关节炎组织形态学和血液流变学的影响  

Effects of Sangsheng Chubi mixture on histology and hemorheology of osteoarthritis in rabbits

作　　者：王平[1] 卢启贵[1] 徐展[1] 孙克民[1] 黄东红[1] 裴代平[1] 

机构地区：[1]深圳平乐骨伤科医院关节病科,广东深圳518010

出　　处：《湖南中医药大学学报》2011年第9期31-35,共5页Journal of Hunan University of Chinese Medicine

基　　金：郑州科技攻关计划项目(郑科计[2004]5号)

摘　　要：目的观察桑生除痹合剂对膝骨性关节炎家兔组织形态学和血液流变学的影响。方法将健康6月龄新西兰大白兔36只,随机分为正常组,模型组,壮骨关节丸组,桑生除痹合剂高、中、低剂量组。每组6只,按照Hulth方法造模,分别灌服高、中、低剂量桑生除痹合剂,壮骨关节丸组灌服壮骨关节丸。12周后,测定血液流变学指标再处死动物,取股骨内侧髁关节负重区软骨及膝关节滑膜标本行光镜观察。结果关节软骨及滑膜标本光镜下呈显著退变表现;壮骨关节丸组和桑生除痹合剂高、中、低剂量组全血黏度、全血还原黏度、血浆黏度及红细胞聚集指数与模型组比较差异有统计学意义(P<0.01)。结论从关节软骨及滑膜的光镜观察可以判断,兔膝骨性关节炎模型建立成功;桑生除痹合剂能明显改善血液流变学状态,改善局部血液循环,延缓软骨退化。Objective To evaluate the effects of Sangsheng Chubi mixture on histology and hemorheology of osteoarthritis in rabbits.Methods 36 rabbits were randomly divided into 6 groups as follows,normal group,model group,control group and three therapeutic groups in high dose,middle dose and low dose respectively,6 rabbits in each group.The models were induced by Hulth method and fed with the mixture in high,middle and low doses;the rabbits in control group fed with Zhuanggu Guanjie pills.After 12 weeks,the rabbits were firstly detected their hemorheology and then killed.Then the histology change of the articular cartilage and synovial membrane were observed with light microscope.Results Degenerations were found in articular cartilage and synovial membrane under light microscope.Compared with model group,the differences of whole blood viscosity,whole blood reduced viscosity,plasma viscosity and erythrocyte aggregation in control group and all therapeutic groups were remarkable（P〈0.01）.Conclusions The osteoarthritis models are successful.Sangsheng Chubi mixture can improve remarkably the hemorheology and the local blood circulation and delay the degenaration of articular cartilage.

关 键 词：膝骨性关节炎 桑生除痹合剂 组织形态学 血液流变学 桑寄生 续断 骨碎补 兔 

分 类 号：R285.5[医药卫生—中药学]