胶体金与蛋白芯片结核抗体检测方法在结核病辅助诊断中的临床价值  

Clinical value of two tuberculosis antibody detection methods in aided diagnosis of tuberculosis

作　　者：刘尚武 李王平[2] 邹远妩 金发光[2] 穆德广[2] 

机构地区：[1]陕西省结核病防治院,西安710100 [2]第四军医大学唐都医院呼吸内科,西安710038

出　　处：《中华肺部疾病杂志（电子版）》2011年第5期30-33,共4页Chinese Journal of Lung Diseases(Electronic Edition)

基　　金：陕西省攻关项目(编号:2010K16-04-08)

摘　　要：目的探讨胶体金与蛋白芯片二种结核抗体检测方法在结核病辅助诊断中的价值。方法收集2010年2月1日至4月30日新入陕西省结核病防治院并同时进行胶体金法及蛋白芯片法检测结核抗体的已确诊或疑诊结核病333例患者的资料,统计二种方法结核抗体检测结果、患者痰菌检测结果以及最后诊断结果,并进行分析。结果二种检测方法结果差异显著,胶体金法敏感性显著高于蛋白芯片法。胶体金法假阳性率为7.21%(24/333),蛋白芯片法38KDa、LAM以及16KDa三种不同抗原检测结果差异较大,以16KDa抗原检出阳性率最低,仅为12.61%(42/333),38KDa、LAM二种抗原检测统计学差异无显著性,但总体敏感性显著低于胶体金法。结论胶体金法检测结核抗体在结核病辅助诊断中的临床价值优于蛋白芯片法。Objective To discuss the value of two tuberculosis antibody detection methods （colloidal gold immunization and protein chip） in aided diagnosis of tuberculosis. Methods To collect data of 333 cases diagnosed or suspected as tuberculosis in Shaanxi TB hospital, whose TB antibody detected with colloidal gold immunization and protein chip meanwhile. It was analyzed that the results of two methods, sputum and final diagnosis. Results Significant difference between two methods were observed. Colloidal gold immunization was more sensitive than protein chip. False positive rate of colloidal gold immunization was 7.21% （24/333）. Significant difference were observed among three antigens, 16 KDa was the lowest, only 12.61% （42/333）. There was no significant difference between antigen 38 KDa and LAM. But the totally sensitivity was lower than colloidal gold immunization. Conculsion The clinical value of aided diagnosis in tuberculosisi with colloidal gold immunization is better than protein chip.

关 键 词：胶体金法 蛋白芯片法 结核抗体 诊断 

分 类 号：R52[医药卫生—内科学]