新型表面印迹材料MIP-P4VP/SiO_2对谷氨酸对映体的手性拆分  

Realizating chiral separation of enantiomers of glutamic acid with novel surface-molecular imprinting technique

作　　者：郭建峰[1] 高保娇[1] 张正国[1] 

机构地区：[1]中北大学化工与环境学院,山西太原030051

出　　处：《化工学报》2011年第11期3207-3214,共8页CIESC Journal

基　　金：山西省自然科学基金项目(2008021013);山西省研究生优秀创新项目(20103085)

摘　　要：引言氨基酸作为构成蛋白质分子的基本单位,是构成人体的最基本物质之一,是生命代谢的物质基础,在人的营养和健康中有着不可替代的作用。在生命科学研究领域。Poly 4-vinyl pyridine/SiO2 （P4VP/SiO2）, a grafted functional particles, was prepared by grafting 4-vinyl pyridine （4VP） on micro-sized silica gel particles with the ＂graft from＂ method, using 3- methacryloxypropyl trimethoxysilane （MPS） as coupling agent. Subsequently, by using the novel surface- molecular imprinting technique, the L-Glu molecule-imprinted material MIP-P4VP/SiO2 was prepared by imprinting towards the grafted P4VP with L-Glu, one enantiomer of glutamic acid （Glu）, as template molecule and 1,6-dibromohexane as crosslinking agent. The recognition characteristics of MIP-P4VP/SiO2 for L-Glu was studied by both static method and dynamic method in which D-Glu was used as a contrast compound. The separation ability of MIP-P4VP/SiO2 towards L-Glu and D-Glu in the racemic s91ution was examined. The experiment results showed that MIP-P4VP/SiO2 had obvious recognition selectivity and fine binding affinity for L-Glu, and the selectivity coefficient was equal to 3.38 with respect to D-Glu, displaying the fine separation property for the enantiomers of glutamic acid. Besides, MIP-P4VP/SiO2 had excellent elution property with diluted aqueous solution of NaOH as eluent, the desorption ratios of L-Glu could reach 99.53% in 12 BV. Moreover, this material showed effective separation ability of repeated use for more than ten times.

关 键 词：谷氨酸 手性拆分 分子表面印迹 4-乙烯基吡啶 

分 类 号：Q819[生物学—生物工程]