几种佐剂对肌肉注射猪支原体肺炎活疫苗免疫刺激能力的增强作用研究  

Immune Study of a Intramuscular Injected Live Vaccine against Mycoplasma hyopneumoniae Enhanced by Different Adjuvants

作　　者：熊祺琰[1] 范志鹏[1,2] 甘源[1] 孔猛[1] 冯志新[1] 刘茂军[1] 邵国青[1] 

机构地区：[1]江苏省农业科学院兽医研究所农业部动物疫病诊断与免疫重点开放实验室国家兽用生物制品工程技术研究中心,江苏南京210014 [2]山西农业大学研究生学院,山西太谷030801

出　　处：《中国畜牧兽医》2011年第10期163-168,共6页China Animal Husbandry & Veterinary Medicine

基　　金：江苏省博士后科研资助计划项目(0902063C);中国博士后科学基金面上资助项目(20090461075);江苏省农业科技创新基金(cx(10)215);农业科技成果转化资金项目(2009FB2C100129);江苏省"333工程"培养资金;"六大人才高峰"资助项目

摘　　要：为增强现有猪支原体肺炎活疫苗的免疫刺激能力以实现肌肉注射免疫,向现有活疫苗中添加几种不同佐剂,考察其经肌肉免疫后机体的免疫应答情况。结果表明,免疫刺激复合物基质佐剂和左旋咪唑+壳聚糖混合佐剂能有效激发细胞免疫应答和体液免疫应答;左旋咪唑+黄芪多糖混合佐剂能增强疫苗的细胞免疫刺激能力,而对体液免疫无作用;皂角素佐剂未显示明显的免疫增强效果。本试验结果为肌肉注射猪支原体肺炎活疫苗的开发奠定了数据基础。To achieve the intramuscular injection of the attenuated live vaccine against Mycoplasma hyopneumoniae,different adjuvants were applied to enhance the immunogenicity of the vaccine.Cellular and humoral immune response was detected after the immunization.The results showed that ISCOM-matrix,as well as the mixture of levamisole and chitosan,effectively increased the proliferation of PBMC and the level of serum IgG antibody.The mixture of levamisole and astragalus polysaccharide only enhanced the cellular immune response while the antibody level showed no change.No obvious enhancement was detected in saponin adjuvant group.These data would help the subsequent researches of the intramuscular inoculating method of this live vaccine.

关 键 词：佐剂 活疫苗 猪支原体肺炎 

分 类 号：S851.3[农业科学—预防兽医学]