rhGH对肠道缺血再灌注损伤大鼠促炎介质介导性肺损伤的影响  

Effects of recombinant human growth hormone(rhGH) on lung injury mediated by the inflammatory mediators associated with gut ischemia-reperfusion injury in rats

作　　者：郄文斌[1] 屠伟峰[1] 彭捷[1] 陈茜[1] 陈朝板[1] 

机构地区：[1]广州军区广州总医院麻醉科全军临床麻醉中心,510010

出　　处：《实用医学杂志》2011年第20期3643-3645,共3页The Journal of Practical Medicine

基　　金：江苏省高校省级重点实验室开放课题(编号:KJS04001)

摘　　要：目的:观察重组人生长激素(rhGH)对肠道缺血再灌注(GIR)损伤大鼠促炎介质介导性肺损伤的影响。方法:42只Wister大鼠随机分为GIR损伤组和治疗组,每组分为缺血前30min,再灌注后48h和72h3个时相点(n=6)。治疗组分别于再灌注后3h和12h使用rhGH向大鼠腹壁皮下注射1U/kg,每间隔12h追加一次。采用夹闭肠系膜前动脉(60min)技术复制GIR损伤大鼠模型。观察各时相点:(1)血浆内毒素(LPS)和肿瘤坏死因子(TNF-α)水平的变化;(2)肺组织中髓过氧化酶(MPO)、弹性蛋白酶(NE)、磷脂酶A2(PLA2)活性的变化;(3)肺组织湿干重比值(W/D)的变化。结果:成功复制了大鼠GIR损伤模型,rhGH用药后可引起:(1)血浆LPS和TNF-α水平与GIR组比较明显降低(P<0.01或P<0.05);(2)再灌注3h和12h用药组肺组织PLA2含量与GIR组比较在再灌注后48h显著下降(P<0.05,P<0.01),其余时象点MPO、PLA2、NE活性与GIR组比较有不同程度变化,但均无统计学意义;(3)再灌注后3h用药组肺组织的W/D与GIR组比较有显著差异(P<0.05)。结论:rhGH能减轻促炎介质介导性肺损伤,可能与降低GIR损伤大鼠血液中LPS和TNF-α的水平和肺组织中PLA2活性有关。Objective To investigate the effects of recombinant human growth hormone （rhGH） on lung injury mediated by the inflammatory mediators associtated with gut ischemia-reperfusion injury in rats. Methods Forty-two Wister rats were randomly allocated to GIR group and treatment group. Each group was redivided into three subgroups （n = 6） according to varying time points ： 30 min before ischemia, 48 h and 72 h after reperfusion. The treatment group was received abdominal wall subcutaneous injection using rhGH 1 U/kg at the time points of 3 h and 12 h after reperfusion, respectively. The treatment was repeated every 12 hours. The rat model of GIR was established by clamping the superior mesenteric artery for one hour. At different time points, plasma levels of LPS and TNF-α, activities of myeloperoxidase （MPO）, neutrophil elastase （NE） and phospholipase A2 （PLA2）, the W/D weight ratio in the lung tissues were determined. Results The mode of GIR was successfully established. Compared with GIR group, the plasma levels of LPS and TNF-α of the administration of rhGH in the 3rd and 12th hour after GIR were significantly decreased （P 〈 0.01, P 〈 0.05）, so was the activity of PLA2 in the lung tissues after 48h post of reperfusion （P 〈 0.05, P 〈 0.01 ）. Compared with GIR group, the W/D weight ratio of the administration of rhGH in the 3rd after GIR was significantly decreased in the lung tissues （P 〈 0.05）. Conclusions RhGH could alleviate lung injury induced by GIR in rats, which may be attributed to the reduction of the plasma levels of LPS and TNF-α, and the inhibition of the PLA2 activity in the lung tissues.

关 键 词：肺损伤 重组人生长激素 再灌注损伤 炎性反应 

分 类 号：R363[医药卫生—病理学]