HPLC法同时测定复方樟脑搽剂中苯酚和樟脑含量  

Simultaneous determination of phenol and camphor in compound camphor liniment by HPLC

作　　者：陆松伟[1] 宋洪杰[1] 陈方剑[1] 

机构地区：[1]第二军医大学长海医院药学部,上海200433

出　　处：《药学实践杂志》2011年第5期347-349,共3页Journal of Pharmaceutical Practice

摘　　要：目的建立同时测定复方樟脑搽剂中苯酚和樟脑含量的HPLC法。方法色谱柱为Kromasil-C18(150 mm×4.6 mm,5μm);流动相为甲醇-水(55∶45);检测波长287 nm;流速0.8 ml/min。结果苯酚和樟脑的线性范围分别在103.4～827.2μg/ml(r=0.999 1,n=5)、205.1～1 640.8μg/ml(r=0.999 5,n=5),平均回收率分别为99.61%(RSD=1.20%,n=9)、99.83%(RSD=1.18%,n=9)。结论该方法简便、准确,重复性好,适用于复方樟脑搽剂的质量控制。Objective To establish a method for simultaneous determination of phenol and camphor in compound camphor liniment by HPLC. Methods The separation was performed on a Kromasil C18 analytical column（ 150 mm × 4.6 mm, 5 μm）with the mobile phase consisting of methanol-water（55 ： 45）. The detection wavelength was 287 nm. The flow rate was 0.8 ml/min. Results The standard curves were linear in the range of 103.4 - 827.2μg/ml for phenol （ r = 0. 999 1, n = 5 ） and 205.1 - 1 640.8μg/ml（ r = 0. 999 5, n = 5 ）for camphor. The average recoveries of phenol and camphor were 99.61% （RSD = 1. 19% , n = 9 ） and 99.83 % （RSD = 1. 18% ,n = 9）. Conclusion The method was simple and accurate with a good reproducibility, and could be used as a quality control method for compound camphor liniment.

关 键 词：高效液相色谱法 复方樟脑搽剂 苯酚 樟脑 

分 类 号：R927[医药卫生—药学]