坡柳种子对脱水干燥的生理生化响应  

Physiological and Biochemical Response of Dodonaea viscose Seeds to Desiccation

作　　者：崔凯[1] 李昆[1,2] 廖声熙[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,昆明650224 [2]国家林业局云南元谋荒漠生态系统定位研究站,昆明650224

出　　处：《林业科学研究》2011年第5期619-626,共8页Forest Research

基　　金：国家十二五科技支撑项目"逆境生态林材料筛选与示范(2011BAD38B01)";科技部林业公益性行业科研专项"201104002-3-3";中国林科院院所长资金"Riricaf200803Z"

摘　　要：为研究超干燥保存手段运用于坡柳种子的可行性,采用正交设计从种子含水量、包装方法、保存温度和预回湿方法4个因素来分析。用硅胶将种子脱水至1.12%6.13%6个含水量梯度,结合不同处理密封保存1年,随后测定发芽率和一些生理生化指标,包括相对电导率(RC)、脯氨酸(Pro)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、可溶性总糖含量和脂肪酸组分。结果表明:种子含水量是对发芽率影响最显著的因素,较为适宜的脱水范围为4.28%5.25%;超干种子发芽率与相对电导率和丙二醛含量呈显著负相关,与脯氨酸含量和3种抗氧化酶活性呈显著正相关;超干种子的油酸和α-亚麻酸含量比对照提高。坡柳种子保存过程中的劣变是由细胞膜损伤和脂质过氧化所引起,而适宜的脱水处理能使抗氧化酶活性增强,使不饱和脂肪酸的含量增加,从而维持种子生活力的稳定。Ultra-dry storage of Dodonaea viscose seeds was studied by orthogonal design including four factors （ seed moisture content, packing method, storage temperature and pre-humidification method）. Seeds were dried to the moisture contents ranging from 1.12% to 6.13% with silica gel, then stored in hermetic condition combined with different treatments for a year. The germination percentage, electrical conductivity （ RC）, proline （ Pro）, malondi- aldehyde （ MDA）, superoxide dismutase （SOD） , peroxidase （POD） , catalase （ CAT）, total soluble sugar content and fatty acid composition of the stored seeds were tested. The results showed that the seed moisture content had sig- nificant effect on germination percentage. The feasible moisture content ranged from 4.28% to 5.25%. The Germination percentage had a significantly negative correlation with the electrical conductivity and proline content, and had significantly positive correlation with the proline content and activity of three antioxidant enzymes. The content of oleic acid and c^-Linolenic acid in ultra - dried seeds were higher compared to the control seeds. It is speculated that the seed deterioration of D. viscose during storage was the result of membrane injury and lipid peroxidation. Suitable ultra-dried treatment, especially in seed moisture content, can increase the activity of antioxidant enzyme and the content of unsaturated fatty acid, which alleviate the seed deterioration.

关 键 词：坡柳 抗氧化酶 脂质过氧化 超干燥保存 

分 类 号：S722[农业科学—林木遗传育种]