葛根芩连片中3种活性成分的HPLC法测定  

Determination of Three Active Ingredients in Gegen Qinlian Tablets by HPLC

作　　者：邓开英[1,2] 王晓琳 夏永鹏 邱宗荫[1] 

机构地区：[1]重庆医科大学,重庆400042 [2]重庆市食品药品检验所,重庆401121 [3]重庆植恩药业有限公司,重庆400041 [4]重庆市卫生局,重庆401147

出　　处：《中国医药工业杂志》2011年第10期784-786,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了高效液相色谱法同时测定葛根芩连片中的葛根素、盐酸小檗碱和黄芩苷。采用C_(18)色谱柱,以甲醇-0.2%磷酸为流动相,梯度洗脱,双波长检测(葛根素为250 nm,盐酸小檗碱和黄芩苷为280 nm)。葛根素、盐酸小檗碱和黄芩苷分别在0.02～0.40μg、0.01～0.20μg和0.01～0.20μg范围内线性关系良好,平均回收率分别为99.2%、97.1%和97.9%,RSD分别为1.7%、2.1%和1.9%。An HPLC method was established for the determination of puerarin, berberine hydrochloride and baicalin in Gegen Qinlian tablets. A C18 column was used with the mobile phase of methanol-0.2 % phosphoric acid by gradient elution at the detection wavelengths of 250 nm for puerarin and 280 nm for berberine hydrochloride and baicalin. The calibration curves were linear in the ranges of 0.02-0.40 μg, 0.01-0.20 μg and 0.01-0.20 μg, respectively. The average recoveries were 99.2%, 97.1% and 97.9%, withRSDs of 1.7%, 2.1% and 1.9%, respectively.

关 键 词：葛根芩连片 葛根素 盐酸小檗碱 黄芩苷 高效液相色谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]