海水淹溺性肺水肿对颅脑创伤后血脑屏障的影响  

Effect of pulmonary edema after seawater drowning on the permeability of blood-brain barrier after draumatic brain injury in rats

作　　者：房文峰[1] 于明琨[2] 

机构地区：[1]南通大学附属无锡第三人民医院神经外科,无锡市214041 [2]中国人民解放军第二军医大学附属长征医院神经外科

出　　处：《江苏医药》2011年第19期2262-2264,I0001,共4页Jiangsu Medical Journal

摘　　要：目的研究海水淹溺性肺水肿对大鼠颅脑创伤后血脑屏障通透性的影响。方法 32只雄性SD大鼠随机均分为海水淹溺(A)组、颅脑创伤合并淡水淹溺(B)组、单纯创伤(C)组和正常对照(D)组。采用侧方液压打击颅脑伤合并海水淹溺大鼠模型,用胶体金示踪血脑屏障通透性的变化。伤后6 h取大鼠伤区脑组织做电镜检查,了解胶体金颗粒透过血脑屏障入脑情况。结果 A组可见神经元细胞线粒体肿胀,髓鞘崩解,胶质细胞肿胀。神经元细胞染色体边集,甚至出现核固缩。脑微血管周围间隙扩大,胞饮活动增强。与B、C组相比,A组电镜可见有更多胶体金颗粒透过血脑屏障入脑。结论海水淹溺性肺水肿可增加血脑屏障的通透性,加重创伤性脑水肿。Objective To investigate the effect of the pulmonary edema after seawater drowning（PE-SWD） on the permeability of blood-brain barrier（BBB） after brain injury in rats.MethodsThirty-two male SD rats were equally randomized into 4 groups of A （seawater drowning）,B（fresh water drowning）,C（brain injury control） and D（blank control）.The brain injury model in groups of A,B and C was established by lateral fluid percussion method and the rats were drowned by injecting seawater（group A） or fresh water（group B） 4 ml/kg intratracheally after brain injury.Colloidal gold（CG） was used to evaluate the permeability of BBB at 6 h after brain injury.The CG particles permeating BBB were observed by electron microscope.Results The neuronal mitochondrial swelling,disintegration of myelin,glial cell swelling,neurons chromosome margination and even nuclear condensation were seen in group A.Space around the brain microvessels was expanded and pinocytosis activity increased as well.Compared to groups of B and C,more CG particles were seen permeating BBB in group A.Conclusion The traumatic brain edema can be exacerbated by seawater drowning-produced increase of BBB permeability in rats.

关 键 词：海水淹溺性肺水肿 创伤性脑损伤 血脑屏障 

分 类 号：R651[医药卫生—外科学]