血培养瓶在眼内炎患者玻璃体标本细菌培养中的应用  

Application of blood culture bottles for culturing vitreous specimen in endophthalmitis cases

作　　者：李娟娟[1] 黎铧[1] 孔蕾[1] 胡竹林[1] 

机构地区：[1]云南省红十字会医院眼科,云南省昆明市650021

出　　处：《眼科新进展》2011年第10期952-953,960,共3页Recent Advances in Ophthalmology

基　　金：云南省科技计划项目:社会发展计划资助(编号:2009ZC175M)~~

摘　　要：目的对比研究采用血培养瓶和传统培养基对眼内炎患者玻璃体标本的细菌培养的方法及效果。方法回顾性分析我院2008年至2010年所诊治的眼内炎患者102例(102眼),平均年龄(35.6±8.5)岁。所有患者均行玻璃体切割术,术中收集玻璃体腔液样本,分别注入血培养瓶和肉汤管(传统培养基)中进行培养,对培养结果及阳性率进行统计分析。结果血培养瓶细菌培养阳性率为60.8%(62例),传统培养基细菌培养阳性率为31.4%(32例)。葡萄球菌是最常见的致病菌,两种培养基阳性率分别为24.19%(15/62)、25.00%(8/32)。结论将玻璃体腔液直接注入血培养瓶中进行细菌培养,操作方便,细菌培养阳性率高于传统培养方法,值得推广。Objective To study the application of blood culture bottles and conventional culture for culturing vitreous specimens in endophthalmitis cases.Methods With 102 eyes of 102 patients with mean age of 35.6 years with endophthalmitis presenting from 2008 to 2010 were retrospectively studied.Vitreous fluid specimens of these patients were cultured in blood culture bottles and conventional culture.The positive rate and culture results were analyzed.Results Vitreous specimens yielded positive was 60.8%（62 cases）with blood culture bottles,31.4%（32 cases）with conventional culture.The most common organism was Staphylococcus epidermidis,the positive rates were 24.19%（15/62） and 25.00%（8/32） in two cultures.Conclusions The direct inoculation of vitreous specimens into the blood culture bottles is easy to perform,with a high yield positive rate.It may be an alternative way to conventional culture media for culturing vitreous specimen in infectious endophthalmitis.

关 键 词：血培养瓶 眼内炎 玻璃体腔液 葡萄球菌 

分 类 号：R771.2[医药卫生—眼科]