益气增敏方对2型糖尿病大鼠骨骼肌组织葡萄糖转运蛋白4表达的影响  

Effects of Chinese herbal medicine Yiqi Zengmin Formula on expression of glucose transporter 4 in skeletal muscle in type 2 diabetic rats

作　　者：薛红丽[1] 张曾[1] 王文健[1] 陈伟华[1] 应健[1] 

机构地区：[1]复旦大学华山医院中西医结合研究所,上海200040

出　　处：《中西医结合学报》2011年第10期1133-1137,共5页Journal of Chinese Integrative Medicine

基　　金：霍英东教育基金会第十一届高校青年教师优选资助课题(No.114036)

摘　　要：目的:研究益气增敏方对高脂饮食联合链脲佐菌素(streptozotocin,STZ)诱导的2型糖尿病(type2diabetes mellitus,T2DM)大鼠骨骼肌细胞葡萄糖转运蛋白4(glucose transporter4,GLUT4)表达的影响。方法:采用高脂饮食联合STZ诱导T2DM建立大鼠模型。健康雄性SD大鼠55只,随机分为对照组(10只)和高脂饲料组(45只),4周后高脂饲料组大鼠腹腔注射STZ(35mg/kg体质量),血糖≥16.67mmol/L者造模成功。将造模成功大鼠随机分为模型组、益气增敏方治疗组、罗格列酮治疗组和氯沙坦治疗组,每组10只。连续灌胃给药8周后,应用蛋白质印迹法检测骨骼肌组织胞浆及胞膜GLUT4蛋白的表达。结果:治疗8周后,与模型组相比,益气增敏方组大鼠GLUT4在胞膜的表达明显增多,在胞浆的表达明显减少(P<0.05);罗格列酮组大鼠GLUT4在胞膜的表达明显增多,在胞浆的表达明显减少(P<0.01);氯沙坦组大鼠GLUT4在胞膜和胞浆的表达无明显变化(P>0.05)。结论:益气增敏方可促进骨骼肌组织GLUT4由胞浆转位至胞膜,有效改善T2DM大鼠的胰岛素抵抗,这一作用与罗格列酮相似。Objective：To investigate the effects of traditional Chinese compound recipe Yiqi Zengmin（YQZM）formula on expression of glucose transporter 4（GLUT4）in skeletal muscle of rats with type 2 diabetes induced by high-fat diet combined with low-dose-streptozotocin injection.Methods：Fifty male rats were randomly divided into two groups：model group fed with high-fat diet（n=40）and control group（n=10）.After 4-week feed of high-fat diet,the rats of the model group were injected with streptozotocin at a dose of 35 mg/kg.Rats with plasma glucose over 16.67 mmol/L were randomly divided into 4 groups：diabetic model group,YQZM group,rosiglitazone group and losartan group and were treated with water,YQZM,rosiglitazone or losartan respectively by oral administration for 8 weeks.The expression of GLUT4 protein in the cytoplasm and the plasma membrane of isolated rats＇ skeletal muscles were detected by Western blotting.Results：The expression of GLUT4 in the plasma membrane was more than that in the cytoplasm in skeletal muscle tissues of the normal rats,while the expression of GLUT-4 in the plasma membrane was less than that in the cytoplasm in skeletal muscle tissues of the type 2 diabetic rats.YQZM formula enhanced GLUT4 translocation from the cytoplasm to the plasma membrane in skeletal muscle tissues,similar to rosiglitazone.Conclusion：YQZM formula can enhance GLUT4 translocation from the cytoplasm to the plasma membrane in skeletal muscle tissues,and displays the insulin sensitization characteristic of rosiglitazone.

关 键 词：复方 2型糖尿病 葡萄糖转运体4 罗格列酮 氯沙坦 大鼠 

分 类 号：R285[医药卫生—中药学]