富血小板血浆提取方法的探讨  

Comparison of the methods for the preparation of platelet-rich plasma

作　　者：王悦[1] 朱喆[2] 刘昕鸣[3] 马英智[2] 周延民[1] 杨旭芳[2] 

机构地区：[1]吉林大学口腔医院种植中心,长春130021 [2]吉林大学白求恩医学院病理系 [3]吉林大学口腔医院第二临床医院检验科

出　　处：《实用口腔医学杂志》2011年第5期643-648,共6页Journal of Practical Stomatology

基　　金：吉林省科学技术厅项目(编号:200804423)

摘　　要：目的:比较不同离心和激活方法处理富血小板血浆(PRP)的效果。方法:1名健康志愿者、30名眼科住院患者静脉采血,分别用PCCSkit法,Curasan法及Trindade法制备PRP。ELISA检测各组血小板衍化生长因子(PDGF)、转化生长因子(TGF-β)、血管内皮细胞生长因子(VEGF),倒置相差显微镜观察PRP添加方式对人成骨样细胞MG63观察效果的影响。结果:Trindade离心法提取的PRP加CaCl2后,PDGF含量高于其他2种方法,PCCSkit法提取的PRP加CaCl2和凝血酶后,TGF-β含量高于其他2种方法,Curasan法提取的PRP加CaCl2后,VEGF浓度高于其他2种方法。3种离心方法中未激活和激活的PRP中生长因子浓度差异有显著性(P<0.05),激活组中CaCl2组和CaCl2加凝血酶组PRP中生长因子含量差异无显著性(P>0.05)。显微镜观察显示,PRP离心后的萃取液可去除RBC对MG63细胞观察效果的影响。结论:3种离心方法间无显著差异;低温、即刻提取PRP,用CaCl2或CaCl2加凝血酶激活均促进生长因子释放;PRP离心萃取液更有利于用于细胞培养中的镜下观察。Objective：To compare the effects of different methods for the preparation of platelet-rich plasma（PRP）.Methods：Venous blood was obtained from a health volunteer and 30 patients of the department of ophthalmology.PRP was prepared by PCCS system,Curasan system and Trindade method respectively.The levels of PDGF,VEGF and TGF-β in the PRP samples were measured using ELISA.A phase contrast microscope was used to observe the morphology of MG63 cells cultured with PRP.Results：PDGF level in the PRP prepared by Trindade method and treated with CaCl2 was higher,TGF-β level in PRP prepared by PCCS and treated with CaCl2 and thrombase was higher,the concentration of VEGF in PRP prepared by Curasan and treated by CaCl2 was higher.CaCl2 increased PDGF level in the PRP prepared by Trindade method,TGF-β by PCCS and Curasan,and VEGF by the three methods（P0.05）,but the two activation methods showed no statistics significance（P0.05）.Centrifuged PRP did not affect the observation of the morphology of cultured MG63 cells.Conclusion：Three methods are effective in the prepariation of PRP.Preparation at low temperature and activation of PRP by CaCl2 or CaCl2 plus thrombin may increase the cytokin content.Centrifuged PRP is suitable for cell culture.

关 键 词：富血小板血浆 生长因子 制备方法 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]