2型糖尿病患者牙槽骨成骨细胞体外培养与生物学特性研究  

Biological feature of alveolar bone osteoblasts from type 2 diabetic patient in vitro

作　　者：孙道才[1] 宋应亮[1] 饶国洲[2] 梁丽华[1] 

机构地区：[1]第四军医大学口腔医学院种植科,西安710032 [2]西安交通大学口腔医学院中心实验室

出　　处：《实用口腔医学杂志》2011年第5期667-671,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81170984)

摘　　要：目的:研究2型糖尿病患者牙槽骨成骨细胞生物学特异性。方法:采用组织块法进行体外培养2型糖尿病患者和正常人牙槽骨成骨细胞,倒置显微镜观察细胞的形态,用碱性磷酸酶(ALP)、茜素红(Alizarinred)染色法分别进行细胞成骨特性的检测,噻唑蓝(MTT)比色法检测细胞的增殖趋势,用酶动力学、放射免疫及酶联免疫分析(ELISA)方法分别进行碱性磷酸酶、骨钙素(BGP)和Ⅰ型胶原(COL-Ⅰ)浓度的检测,结果:在同等培养条件下,二者的形态无明显差异,但2型糖尿病患者成骨细胞较正常人成骨细胞生长慢、活性弱,矿化结节形成少;2型糖尿病成骨细胞上清液中ALP、BGP及COL-Ⅰ浓度均较正常人低(P<0.05)。结论:2型糖尿病患者牙槽骨成骨细胞增殖较缓慢,特征性分泌物含量较正常成骨细胞低。Objective： To study the biological feature of alveolar bone osteoblasts from type 2 diabetic patient. Methods： Osteoblasts from a type 2 diabetic patient and a healthy subject were respectively cultured in vitro with tissue explants adherent method. The morphology of the cells was observed under phase contrast microscope. MTT assay,ALP staining, Alizarin red staing, ALP, BGP and COL-Ⅰconcentration examinations, radio-immunoassay and ELISA method were performed to detect the osteogenic characteristics of the cells. Results： The cells of the 2 groups showed no significant difference in morphology. However, the alveolar bone osteoblasts from type 2 diabetic patient had slower proliferation, lower activity of calcium nodule formation, lower concentration of ALP, BGP and COL-1 in supernatant of cell culture than those from normal control（P0.05）. Conclusion： The alveolar bone osteoblasts of type 2 diabetic patients can be cultured in vitro, but with slower proliferation and lower osteogenic activity.

关 键 词：种植 成骨细胞 2型糖尿患者 牙槽骨 细胞培养 

分 类 号：R587.1[医药卫生—内分泌] R783.5[医药卫生—内科学]