水稻SUPERMAN类似基因ZOS11-11的表达分析及功能初探  

Expressional Analysis and Preliminarily Functional Research of a SUPERMAN-like Gene ZOS11-11 in Rice (Oryza sativa L.)

作　　者：李生平[1] 段远霖[1,2] 刁志娟[1] 庄丽君[1] 吴为人[1,2] 

机构地区：[1]教育部作物遗传育种与综合利用重点实验室,福建农林大学,福州,350002 [2]福建省水稻分子标记辅助育种重点实验室,福建农林大学,福州350002

出　　处：《分子植物育种》2011年第5期531-535,共5页Molecular Plant Breeding

基　　金：国家自然科学基金(30671122);国家863计划(2006AA10Z128);福建省自然科学基金(B0620001)共同资助

摘　　要：SUPL(SUPERMAN-like)是一类单C2H2型锌指蛋白,SUPL基因在植物生长发育过程中起着重要的调控作用。利用生物信息学分析方法,我们从水稻中找到了一个SUPL基因ZOS11-11,它位于水稻第11号染色体上,编码245个氨基酸。序列分析结果表明,ZOS11-11具有与其它SUPL蛋白相同的功能结构域,包括N端高度保守的"QALGGH"序列、核定位信号序列以及C端具有转录调节功能的EAR结构域。定量PCR与GUS表达分析表明,ZOS11-11几乎在水稻所有的组织、器官中都有表达,其中在根、茎、叶和雌雄蕊分化阶段的幼穗中表达较强,而在其它发育阶段的穗中表达相对较弱,表明ZOS11-11可能参与了水稻生长发育的调控。但是,增加ZOS11-11的表达量并没有明显影响水稻的生长发育。这些结果为ZOS11-11基因功能的深入研究奠定了基础。SUPL （SUPERMAN-like） is a kind of single C2H2 zinc finger genes, which play important roles in the growth and development of plants. By bioinformatics analysis, we identified a SUPL gene ZOS11-11 in rice, which is located in chromosome 11, encoding a protein of 245 amino acids. Sequence analysis showed that ZOS11-11 has the same functional domain as in other SUPL proteins, including the ＂QALGGH＂ sequence in the N terminal, the nuclear localization signal sequence, and the EAR domain with transcription regulating function in the C terminal. Quantitative PCR and GUS expression analyses showed that ZOS11-11 is expressed almost in all tissues and organs, with strong expression in root, stem, leaf and young panicles at the stage of pistil and stamen development and weak expression in panicles at other developmental stages, implying that ZOS11-11 might be involved in the regulation of vegetative growth and development in rice. However, over-expression of ZOS11-11 did not exhibit obvious influence on rice growth and development. These results make a basis for further functional analysis of ZOS11-11.

关 键 词：水稻(Oryza SativaL.) ZOS11-11 锌指基因 表达 功能 

分 类 号：S511[农业科学—作物学]