小麦南大2419及其衍生品种(系)主要农艺性状的关联分析  

Association Analysis of Main Agronomic Traits in Wheat of Nanda2419 and Its Derivatives

作　　者：赖运平[1] 李俊[1] 刘新春[2] 彭正松[3] 胡晓蓉[1] 杨武云[1] 

机构地区：[1]四川省农业科学院作物研究所,成都610066 [2]四川农业大学农学院,温江611130 [3]西华师范大学生命科学学院,南充637002

出　　处：《分子植物育种》2011年第5期536-546,共11页Molecular Plant Breeding

基　　金：国家自然科学基金项目(30871532);国家973计划项目(2006CB101700);现代农业产业技术体系建设专项资金;四川省育种攻关项目;四川省国际合作项目(2010HH0052;2011HH0026)共同资助

摘　　要：筛选与小麦重要农艺性状相关联的SSR标记,对小麦分子标记辅助育种有重要的实践意义。本研究利用多态性较高的80个SSR标记,对南大2419及其71份衍生后代品种(系)进行基因型分析,采用TASSEL软件的MLM(Mixed linear model)方法对籽粒产量、千粒重、有效穗、穗粒数等8个主要农艺性状进行SSR标记与性状的关联分析。结果表明:(1)该群体由6个亚群组成;(2)群体SSR数据分析发现,无论是连锁还是非连锁SSR位点组合都存在不同程度的连锁不平衡(Linkage disequilibrium,LD);在各基因组选择标记数基本相同的情况下,D基因组中显著LD位点比例(8.9%)明显低于A基因组(15.3%)和B基因组(13.8%),但D基因组的LD水平较高;(3)群体中共20个SSR位点与8个农艺性状显著关联,4个位点2年被检测到与同一性状显著相关(p<0.01),10个位点位于家系连锁定位的QTL区间或附近,多数位点/性状关联结果与前人研究一致;13个标记位点同时与2个或多个性状关联。To make advances in wheat molecular marker-assisted breeding, it is important to identify microsatellite markers associated with agronomical traits. In this study, eight agronomic traits and 80 microsatellites were used to evaluate a population with derivatives of Nanda2419. Population structure and linkage disequilibrium （LD） of loci were analyzed for the population, and then the association analysis between SSR loci and main agronomic traits including grain yield, spikes per square meter and grain number per spike, were performed by using TASSEL software MLM （Mixed linear model） program. The result showed followings： （1） Genetic structure analysis showed that 71 accessions were composed of six subpopulations. （2） SSR data analysis revealed that the different degrees of LD were detected not only among linked markers but also among unlinked ones. The D genome had lower proportion of markers in significant LD （8.9%） than A （15.3%） and B （13.8%） genomes, even though it had the same amount of makers coverage. But they were the highest degrees of LD for D genome. （3） There are 20 SSR loci associated with 8 agronomic traits were screened out. Among those, 4 marker loci were found to associate with the same trait and 10 SSR loci were located on the regions which were mapped by family-based linkage mapping procedure of previous authors. A number of loci associated with traits in the present paper are consistent with previous studies. There were 13 SSR maker loci associated with two or more traits simultaneously.

关 键 词：南大2419 SSR 群体结构 连锁不平衡 关联分析 

分 类 号：S512.1[农业科学—作物学]