离子束介导大豆DNA的小麦变异株系灌浆后期蛋白水解酶、POD表达分析  

Analysis of Proteinases and Prioxidases in Different Wheat Mutant Lines with Soybean DNA by Ion Beam during Later Grain Filling

作　　者：李江伟[1,2] 赵一丹[1] 朱德来[3] 魏松浩[3] 

机构地区：[1]河南农业大学农学院,郑州450002 [2]新乡市农科院,新乡453003 [3]河南师范大学生命科学学院,新乡453007

出　　处：《分子植物育种》2011年第5期567-571,共5页Molecular Plant Breeding

基　　金：河南省自然科学基金(082300430360)资助

摘　　要：本研究用蛋白水解酶复性电泳技术,分析了离子束介导大豆DNA的两个高蛋白小麦变异株系和两个低蛋白小麦变异株系旗叶和倒二叶在灌浆后期蛋白水解酶、POD的种类。结果表明,变异株系和对照间以及变异株系间蛋白水解酶、POD的种类和活性存在显著差异;变异株系中检到了一些新的蛋白水解酶带,在pH5.0条件下变异株系C倒二叶检出220kD一条新酶带;pH7.0时变异中性B检到220kD一条新酶带,株系C中检到260kD一条新酶带;在pH8.5时,在变异中A、B、C检到220kD新酶带,在变异株系B中检到260kD和280kD两条新酶带。POD表达酶谱中也发现新带和缺失酶带,变异株系A的旗叶中检到37kD、39kD和212kD共3条新带,变异株系B缺失42kD和47kD两条酶带。这些新酶带为进一步研究变异株系在DNA水平的差异积累资料。By renaturation electrophoresis, we analyzed the bands of neutral proteinases and POD isoenzyme in flag leaf and the second leaf above leaf in two high protein and two low protein mutant wheat lines by transferring the total DNA of bean by ion beam. Results showed that there were apparently differences of kinds and activities between wide type and mutant lines. The new proteinase bands had been measured including a 220 kD band in lines C second leaf above at pH 5.0, a 220 kD band in line B and a 260 kD band in line B at pH 7.0. A new band at 220 kD was found in all A, B and C lines, meanwhile a 260 kD and 280 kD new bands was only found in line B. As to POD both new and deletion bands were checked in mutant lines. Three bands at 37 kD, 39 kD and 212 kD were new appearance in line B and two bands at 42 kD and 47 kD were disappeared in line B. Our results are of significance to further research the difference in DNA level in mutant lines.

关 键 词：蛋白水解酶 过氧化物酶 离子束 小麦 大豆DNA 

分 类 号：S512.1[农业科学—作物学]