Identification and characterization of a cytochrome P450 C YP6CX1 putatively associated with insecticide resistance in Bemisia tabaci  

作　　者：Hua-Mei Zhuang Kuan-Fu Wang Lin Zheng Zu-Jian Wu Tadashi Miyata Gang Wu 

机构地区：[1]Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China [2]Laboratory of Applied Entomology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan

出　　处：《Insect Science》2011年第5期484-494,共11页昆虫科学（英文版）

摘　　要：The novel full length of cytochrome P450 gene has been isolated in insecticideresistant （named CYP6CXlvl） and -susceptible （named CYP6CXlv2） Bemisia tabaci, which was identified as B biotype, in Shangjie, Fujian, China （Sj）. CYP6CX1 （1 940 bp contained a 1 557 bp open reading frame） included conserved domains common to CYP6 members, such as heme-binding motif PFGEGPRFCIA, putative ＂meander＂-binding sequence ETLR and PERF in helix-K, oxygen-binding motif AGLDPV and conserved sequence PEKFNP near the carboxyl end. There were four different replacements of amino acid residues between R and S B. tabaci （Thr300 Ala, Thr354Pro, Arg486His and Ile503Thr）, among which the substitution Ile503Thr was located in the substrate recognition sites region. The mRNA transcription level of CYP6CXlvl was 2.38-fold as high as that of CYP6CXlv2. The results indicated that the CFP6CX1 from the B biotype B. tabaci in Sj was one of the CYP6 members, and enhanced CYP6CX1 expression and substitute of amino acid residues might be involved in the resistance mechanisms in field B. tabaci.The novel full length of cytochrome P450 gene has been isolated in insecticideresistant （named CYP6CXlvl） and -susceptible （named CYP6CXlv2） Bemisia tabaci, which was identified as B biotype, in Shangjie, Fujian, China （Sj）. CYP6CX1 （1 940 bp contained a 1 557 bp open reading frame） included conserved domains common to CYP6 members, such as heme-binding motif PFGEGPRFCIA, putative ＂meander＂-binding sequence ETLR and PERF in helix-K, oxygen-binding motif AGLDPV and conserved sequence PEKFNP near the carboxyl end. There were four different replacements of amino acid residues between R and S B. tabaci （Thr300 Ala, Thr354Pro, Arg486His and Ile503Thr）, among which the substitution Ile503Thr was located in the substrate recognition sites region. The mRNA transcription level of CYP6CXlvl was 2.38-fold as high as that of CYP6CXlv2. The results indicated that the CFP6CX1 from the B biotype B. tabaci in Sj was one of the CYP6 members, and enhanced CYP6CX1 expression and substitute of amino acid residues might be involved in the resistance mechanisms in field B. tabaci.

关 键 词：Bemisia tabaci BIOTYPE cytochrome P450s monooxygenases insecticide resistance 

分 类 号：S482.39[农业科学—农药学] Q785[农业科学—植物保护]