发状念珠藻藻蓝蛋白分离纯化及性质研究  

Purification and Characterization of C-phycocyanin from Liquid Cultured Nostoc flagelliforme Cells

作　　者：岳思君[1,2] 贾士儒[1] 丁文杰[1] 戴玉杰[1] 

机构地区：[1]天津科技大学,工业微生物教育部重点实验室,天津300457 [2]宁夏大学生命科学学院,宁夏银川750021

出　　处：《食品与发酵工业》2011年第9期1-6,共6页Food and Fermentation Industries

基　　金：国家自然科学基金(20776112,20376061);国家“十一五”支撑计划项目(2006BAD27B06)

摘　　要：采用冻融法破碎发状念珠藻细胞,经过磷酸缓冲液提取、硫酸铵分步沉淀、离子交换层析和凝胶层析分离纯化发状念珠藻藻蓝蛋白,其纯度(A615/A280)达到5.321,纯化因子为7.623。纯化的藻蓝蛋白最大吸收峰为615 nm,其室温荧光发射峰为646 nm,SDS-PAGE电泳显示其α和β亚基的分子质量在14.4～18.4 ku。稳定性实验表明,低温、避光、pH 6～8以及低浓度的蔗糖等条件下发状念珠藻藻蓝蛋白是稳定的。实验结果将为开发利用发状念珠藻藻蓝蛋白提供参考。It＇s useful measure for the protection of the endangered natural resources to extract useful substances from artificial cultured species. In this study, C-phycocyanin was extracted from liquid-cultured Nostoc flagelliforme cells with repeated frost thawing followed by frozen ultrasonic method. The crude extract was successfully purified by procedure of ammonium sulfate precipitation, ion exchange chromatography with DEAE 650M column, and gel filtration chromatography with Sephadex G200 column. The purity ratio and purity factor of the purified C-phycocyanin reached 5. 321 and 7. 623 respectively after successive purification procedure. It has a maximum absorption at 615nm and a maximum fluorescence emission at 646nm. There are two bands appeared at about 15.7ku and 16.8 ku for SDS-PAGE, which are attributed to α and βsubunits of C-phycocyanin. The stability tests indicated that C-phyeoeyanin is stable in the range pH 6 to 8 with temperature below 30℃ in the dark or indoor light conditions. These results help the further study on exploitation of C-phycocyanin from N. flagelliforme cells.

关 键 词：发状念珠藻 藻蓝蛋白 离子交换层析 凝胶层析 

分 类 号：Q946[生物学—植物学]