高效液相色谱法测定人血浆中奥卡西平活性代谢物利卡西平的浓度  

HPLC determination of active metabolite of oxcarbazepine-licarbazepine in human plasma

作　　者：何益锋[1] 程钢[1] 周述香[1] 曾玲[1] 潘霞云[1] 

机构地区：[1]湖南省常德市第一人民医院,湖南常德415003

出　　处：《中南药学》2011年第9期683-686,共4页Central South Pharmacy

摘　　要：目的建立反相高效液相色谱法测定人血浆中奥卡西平（OXC）活性代谢物利卡西平（MHD）的浓度。方法以苯巴比妥为内标,血样用乙醚萃取;采用SHIMADZU VP-ODS（150 mm×4.6 mm,5μm）色谱柱,流动相为乙腈-甲醇-0.05 mol.L-1磷酸二氢钾缓冲液-四甲基乙二胺（20∶19∶61∶0.1,用磷酸调pH=6.0）,流速0.8 mL.min^-1,柱温为30℃,二极管阵列检测器检测波长232 nm。结果血浆中内源性杂质对样品测定无干扰,MHD在1.0-80.0μg.mL-1线性关系良好。MHD日内、日间RSD均〈9%,平均萃取回收率均〉66%,方法回收率为87%-98%。结论本法简便、灵敏、准确,适用于人体内奥卡西平活性代谢物MHD的血药浓度监测。Objective To establish an HPLC method for the determination of active metabolite of oxcarbazepine（OXC）-li-carbazepine（MHD） in human plasma.Methods Phenobarbitol was used as the internal standard（IS）.The samples were extracted by diethyl ether and the diode array detection wavelength was 232 nm.RP-HPLC was performed on a SHIMADZU VP-ODS（150 mm×4.6 mm,5 μm） column at 30 ℃.The mobile phase consisted of acetonitrile-methanol-0.05 mol·L-1 potassium dihydrogen phosphate buffer solution-tetramethylethylenediamine（20∶19∶61∶0.1,pH=6.0） at 0.8 mL·min^-1.Results The drug-free plasma did not interfere with the determination of drugs and the internal standard.The calibration curve of MHD was linear over 1.0-80.0 μg·mL^-1,with the correlation coefficient of 0.999 6.Inter-day and intra-day RSD were less than 9%.The average extraction recoveries were all above 66% and the methodology recoveries were between 87% and 98%.Conclusion The method is simple,sensitive and accurate.It is suitable for the determination of active metabolite of OXC-MHD in human plasma.

关 键 词：奥卡西平 利卡西平 活性代谢物 高效液相色谱法 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R927.2[医药卫生—药学]