五倍子对鳗鲡4株常见病原菌的体外抗菌后效应  

Postantibiotic Effect of Galla chinensis on Pathogenic Bacteria of Eels in vitro

作　　者：李忠琴[1] 关瑞章[1] 韩金钢[1] 汪黎虹[1] 刘宏伟[1] 

机构地区：[1]集美大学水产学院教育部鳗鱼现代产业技术工程研究中心,福建厦门361021

出　　处：《中国兽药杂志》2011年第5期1-4,共4页Chinese Journal of Veterinary Drug

基　　金：农业部科研专项(nyhyzx07-043);福建省科技计划重点项目(2009N0046);福建省教育厅重点资助项目(JA09157);福建省科技厅平台建设子课题(2009N2003-1);集美大学创新团队基金(2010A003);集美大学博士科研启动基金(ZQ2008015);集美大学优秀青年骨干教师基金(2008C001)

摘　　要：采用试管液体二倍稀释法测定中药五倍子对鳗鲡养殖中常见主要致病菌（肠杆菌B01、迟缓爱德华氏菌B09、豚鼠气单胞菌B18和嗜水气单胞菌B27）的最小抑菌浓度（MIC）。然后再采用菌落计数法分别测定五倍子在0.25、0.5、1、2、4倍于各菌MIC时对鳗鲡病原菌株B01、B09、B18、B27的体外抗菌后效应（PAE）。结果显示：五倍子在0.25、0.5、1、2、4倍MIC值时对鳗鲡病原菌株B01、B09、B18、B27均具有一定的体外抗菌后效应,且PAE与药液浓度在一定范围内（0.25~4倍MIC）呈剂量依赖性,当药液浓度达4倍MIC时,PAE明显延长（P〈0.05）;五倍子对各病原菌株B01、B09、B18、B27的PAE值在1倍MIC浓度以上差异显著（P〈0.05）,在4倍MIC时对于病原菌株B01和B18的PAE之间差异较小。结论：实验结果提示在养殖鳗鲡病害防治中设计投药方案时,对主要致病菌B01、B09、B18和B27感染所造成的烂鳃、肝胆肿大、败血症等疾病,除考虑药代动力学和MIC指标外,还应考虑PAE因素,可适当延长给药间隔时间,降低药物对鳗鲡的副作用及对其摄食量和生长的负面影响。The postantibiotic effect（PAE） of Galla chinensis on cardinal pathogenic bacteria of eels including of Enterobacter B01,Edwardsiella tarda B09,Aeromonas caviae B18,Aeromonas hydrophila B27 in vitro were determined in this study.Dilution method was used for the drug of Galla chinensis removal and Mueller-Hinton agar colony counting method was used to monitored the growth of bacteria.The results showed that marked PAE was found even in the subinhibitory concentration（sub-MIC） when Galla chinensis were used against four common pathogenic bacteria of eels and the isolate obtained from veterinary clinic at concentration of 0.25,0.5,1,2 and 4 times of MIC.The duration of PAE was enhanced with the increasing concentration,showing strongly a concentration dependent,especially at 4 times of MIC,the PAE was obvirously prolonged（P0.05）.It suggested that longer dosing interval should be recommended when the Galla chinensis drug used against eels pathogenic bacteria,which achieved reducing side-effect against eels and reversely working on growth of eels.

关 键 词：五倍子 鳗鲡 病原菌 抗菌后效应 

分 类 号：S853.7[农业科学—临床兽医学]