猪K_(88)、K_(99)、987P、F_(41)埃希氏大肠杆菌高密度发酵培养技术的初步探讨  

Study on High Density Fermentation of Escherichia coli K_(88),K_(99),987P and F_(41)

作　　者：巫月生 齐冬梅 张毓金 赖月辉 李嘉爱 王卓明 

机构地区：[1]广东永顺生物制药有限公司,广州萝岗511356

出　　处：《中国兽药杂志》2011年第5期8-10,共3页Chinese Journal of Veterinary Drug

摘　　要：采用高密度发酵和普通深层通气两种方法培养含K88、K99、987P、F41菌毛抗原的四株猪埃希氏大肠杆菌,对其培养液进行活菌数、OD值、pH值、效价的测定。实验结果表明,运用高密度发酵方法培养,各菌活菌数可达4.1×1010~4.9×1010CFU/mL,效价为211~213;运用普通深层通气方法培养,各菌活菌数为0.51×1010~0.59×1010CFU/mL,效价为24~25,可选择高密度发酵方法替代普通深层通气方法培养,用于制备猪埃希氏大肠杆菌K88、K99、987P、F41四价菌毛提纯苗。Culture Escherichia coli fimbiral antigen K88,K99,987P AND F41 by bioreactor and culture-bottles,and then measure their viable count（colony forming unit）,OD,pH and titer.The experimental results show that viable count reached 4.1×1010～4.9×1010 CFU/mL and titer reached 211～213 when bacteria were cultured by bioreactor.Viable count reached 0.51×1010～0.59×1010 CFU/mL and titer reached 24～25 when bacteria were cultured by culture-bottles.The results suggest that bioreactor can be used to culture Escherichia coli fimbirial antigen K88,K99,987P and F41.

关 键 词：猪埃希氏大肠杆菌 K88、K99、987P、F41 菌毛抗原 高密度发酵培养 

分 类 号：S852.61[农业科学—基础兽医学]