5-羟色胺转运体基因多态性与女性围绝经期及绝经后期首发抑郁障碍的关联研究  

Study on the correlation between morphology of 5-hydroxy tryptamine transporter gene and the first onset of depressive disorder in women during perimenopausal period and postmenopausal period

作　　者：顾红亮[1] 陶晶[2] 仇剑崟[2] 杨慧琳[1] 陈静[2] 王祖承[2] 

机构地区：[1]上海市浦东新区妇幼保健院,201206 [2]上海交通大学医学院附属精神卫生中心

出　　处：《中国妇幼保健》2011年第28期4377-4381,共5页Maternal and Child Health Care of China

基　　金：高等学校博士学科点专项科研基金〔20050266006〕

摘　　要：目的:研究5-羟色胺(5-HT)转运体基因多态性与女性围绝经期及绝经后期首发抑郁障碍的关联,以探索此时期抑郁障碍发生的遗传易感基因。方法:通过病例-对照研究设计,纳入63例围绝经期及绝经后期首发抑郁障碍汉族女性患者为病例组,131例围绝经期及绝经后期健康汉族女性为对照组,应用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)方法,进行5-HT转运体基因启动子区域(5-HTTLPR)功能三等位多态位点及其第2内含子上的可变数目串联重复序列多态位点Stin2的基因分型。结果:5-HTTLPR功能三等位多态各基因型和等位基因频率分布在病例组及对照组差异具有统计学意义(P<0.05),LA等位基因和LALA基因型为此时期抑郁障碍患病的危险等位基因和基因型。Stin2位点各基因型和等位基因频率分布在病例组及对照组差异无统计学意义(P>0.05)。5-HTTLPR与Stin2位点构建的单体型中L12单体型与患病存在关联。结论:5-HTT基因多态性与围绝经期及绝经后期抑郁障碍的发生存在关联。Objective：To study the correlation between morphology of 5-hydroxy tryptamine（5-HT） transporter gene and the first onset of depressive disorder in women during perimenopausal period and postmenopausal period,explore the genetic susceptibility gene of depressive disorder.Methods：A case-control study was conducted,63 female patients of Han nationality with the first onset of depressive disorder during perimenopausal period and postmenopausal period were selected as case group,131 healthy women of Han nationality during perimenopausal period and postmenopausal period were selected as control group,PCR-RFLP was used to detect the genotypes of functionally triallelic morphological locus in 5-HTTLPR and variable number tandem repeat（VNTR） morphological locus（Stin2） in the second intron of 5-HTTLPR.Results：There was significant difference in frequency distributions of genotypes and alleles of functionally triallelic polymorphism of 5-HTTLPR between case group and control group（P0.05）,LA allele and LALA genotype were the risk allele and genotype for the patients with depressive disorder during perimenopausal period and postmenopausal period.There was no significant difference in frequency distributions of genotypes and alleles of Stin2 locus between case group and control group（P0.05）.There was a correlation between L12 haplotype constructed by 5-HTTLPR and Stin2 locus and the occurrence of depressive disorder.Conclusion：There is a correlation between 5-HTT gene polymorphism and the occurrence of depressive disorder during perimenopausal period and postmenopausal period.

关 键 词：围绝经期 绝经后期 抑郁障碍 5-羟色胺转运体基因 关联研究 

分 类 号：R711.75[医药卫生—妇产科学]