香港海鸥形菌荧光PCR快速检测方法的建立  

The development of real-time PCR assay for the rapid detection of Laribacter hongkongensis

作　　者：邱亚群[1] 石晓路[1] 扈庆华[1] 林一曼[1] 李迎慧[1] 吕东月[1] 

机构地区：[1]广东省深圳市疾病预防控制中心,广东深圳518055

出　　处：《中国卫生检验杂志》2011年第10期2368-2370,共3页Chinese Journal of Health Laboratory Technology

基　　金：深圳市科技局(201003125)

摘　　要：目的:建立检测香港海鸥形菌的实时荧光PCR方法。方法:根据香港海鸥形菌以16SrDNA基因设计探针和引物,建立检测香港海鸥形菌荧光PCR方法,并对建立的方法进行特异性、灵敏性的评价。结果:检测体系灵敏度高,菌液的最低检出限可达40 CFU/反应体系;特异性好,对329株细菌的检测符合率达100%。结论:建立的实时PCR检测方法可应用于食源性致病菌中香港海鸥形菌的快速检测。Objective：To develop a real-time PCR assay for the rapid detection of Laribacter hongkongensis.Methods： Based on the sequences of 16SrDNA gene published on GeneBank,primers and molecular beacon probe were designed,the real-time PCR assay for the rapid detection of Laribacter hongkongensis was developed.Then the specificity,sensitivity of the real-time PCR method were evaluated.Results： The detection system had high sensitivity and the lowest detection limit was 40CFU/reaction.There was no cross-reaction with other bacteria as control.The assay was tested against 329 strains,no false signals were observed and the result was consistence with the traditional method.Conclusion： The real-time PCR assay could be applied to the rapid detection of Laribacter hongkongensis on the foodborne pathogens.

关 键 词：香港海鸥形菌 实时荧光PCR 

分 类 号：R378.99[医药卫生—病原生物学]