云南边防部队腹泻患者中8株豚鼠气单胞菌的分离鉴定及致病性研究  

Research on isolation,identification and pathogenicity of 8 strains of aeromonas caviae in soldiers with diarrhea in border forces of Yunnan

作　　者：王意银[1,2] 李刚山[2] 邓波[1,2] 朱姝媛[3] 朱琼媛[4] 赵丽芝[5] 王惠萱[6] 张朝雄[2] 周丽华 张富强[2] 黄留玉 熊鸿燕[1] 范泉水[1,2] 

机构地区：[1]第三军医大学军事预防医学院军队流行病学教研室,重庆400038 [2]成都军区疾病预防控制中心,昆明650032 [3]成都军区第三门诊部,昆明650032 [4]云南省第二人民医院,昆明650031 [5]昆明市第一人民医院,昆明650000 [6]成都军区昆明总医院,昆明650032 [7]云南省林业医院,昆明650224 [8]解放军疾病预防控制所,北京100071

出　　处：《中国卫生检验杂志》2011年第10期2439-2440,共2页Chinese Journal of Health Laboratory Technology

基　　金："十一五"国家科技重大专项(2009ZX10004-205)

摘　　要：目的:对豚鼠气单胞菌分离株进行鉴定,掌握豚鼠气单胞菌在驻云南边防部队引起腹泻的情况。方法:碱性蛋白胨水增菌,TCBS、庆大霉素或碱性琼脂平板分离培养,进行系统生化鉴定,电子显微镜观察形态。小白鼠腹腔注射法测定其毒力,开展致病性研究。药敏试验采用纸片扩散法。结果:从502份腹泻患者粪便标本中检出8株豚鼠气单胞菌。结论:分离的8株豚鼠气单胞菌具有较强毒力,与致病性密切相关。药敏试验可作为此菌感染时临床用药的重要参考。Objective：To survey the infection staus of Aeromonas caviae in soldiers in Yunnan Border army through identification of the separated strains of Aeromonas caviae.Methods： The bacteria were sired in Alkaline peptone water,cultured and isolated on TCBS or Gentamicin or Alkaline agar plate,then the bacterial pure cultures were indentified by systemic biochemical test.The morphology of the bacteria was observed under microscope.In addition,the pathogenesis of the bacteria were investigated by intraperitoneal injection in mice and Susceptibility test were analyzed by Disk diffusion method.Results： 8 strains of aeromonas caviae were found out from 502 stool specimens of patients with diarrhea.Conclusion： The Aeromonas caviae is associated with Pathogenicity.Susceptibility test can be used as an important reference for clinical use in aeromonas caviae infection.

关 键 词：腹泻 豚鼠气单胞菌 致病性 药敏试验 

分 类 号：R378.3[医药卫生—病原生物学]