三氯乙烯变态反应病人外周血凋亡基因表达水平的研究  

Study on mRNA expression of apoptosis genes in patients with allergic disorder induced by trichloroethylene

作　　者：周丽[1] 徐新云[1] 刘月峰[1] 黄新凤[1] 杨细飞[1] 毛吉炎[1] 毛侃琅[1] 

机构地区：[1]广东省深圳市疾病预防控制中心,广东深圳518055

出　　处：《中国卫生检验杂志》2011年第10期2455-2457,共3页Chinese Journal of Health Laboratory Technology

基　　金：深圳市科技计划项目资助课题(200802073)

摘　　要：目的:探讨三氯乙烯(TCE)变态反应病人外周血凋亡基因BAX、BAD、Bcl-2、Caspase-3、Caspase-8及Caspase-9的mRNA表达水平变化情况。方法:抽取健康人(对照组)和三氯乙烯变态反应病人(病例组)抗凝外周全血,采用实时荧光定量PCR(real-time fluorescent quantitative PCR)技术检测外周血中BAX、BAD、Bcl-2、Caspase-3、Caspase-8及Caspase-9的mRNA的表达水平。结果:TCE变态反应病人(病例组)BAX、BAD、Bcl-2基因mRNA表达水平与对照组相比较,分别升高85%1、39%、227%(P<0.01);病例组Caspase-3、Caspase-8、Caspase-9基因mRNA表达水平也比对照组分别升高130%、231%2、49%(P<0.01)。结论:三氯乙烯病人外周血凋亡基因表达水平明显高于对照组,提示这些凋亡基因可能与TCE引起的变态反应存在一定的关联。Objective：To study mRNA expression of apoptosis genes BAX,BAD,Bcl-2,Caspase-3,Caspase-8,Caspase-9 in peripheral blood of patients with allergic disorder induced by trichloroethylene（TCE）.Methods： Peripheral blood samples were collected from the healthy workers（control） and TCE patients.Real-time fluorescent quantitative PCR was used to detect mRNA expression of BAX,BAD,Bcl-2,Caspase-3,Caspase-8,Caspase-9.Results：The levels of BAX,BAD,Bcl-2 mRNA expression increased by 85%,139%,227%,respectively in TCE patients when compared with the control group,the differences between TCE patients and control were significant（P〈0.01）.Additionally,Caspase-3,Caspase-8,Caspase-9 mRNA expression increased by 130%,231%,249%,respectively in TCE patients when compared with the control group（P〈0.01）.Conclusion：Trichloroethylene induced alteration of apoptosis gene mRNA expression in patients with allergic disorder,these findings indicated that some apoptosis genes might play important roles in TCE-induced allergy.

关 键 词：三氯乙烯 变态反应 凋亡基因 实时荧光定量PCR 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]