A near-infrared fluorescent probe for fluorine ions and its application in the imaging of HepG2 cells  

作　　者：GONG Wei SU RuiXian LI Lu XU KeHua TANG Bo 

机构地区：[1]Key Laboratory of Molecular and Nano Probes of Ministry of Education, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production of Ministry of Education, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China

出　　处：《Chinese Science Bulletin》2011年第31期3260-3265,共6页

基　　金：supported by the National Basic Research Program of China (2007CB936000);the National Natural Science Foundation of China (20875057);the National Natural Science Funds for Distinguished Young Scholar (20725518);the National Key Natural Science Foundation of China (21035003);the Science and Technology Development Programs of Shandong Province of China (2008GG30003012);the Key Natural Science Foundation of Shandong Province of China (ZR2010BZ001)

摘　　要：In this study, we have designed and synthesized a new near-infrared (NIR) fluorescent probe (BODIPY-Se) to detect fluorine ions (F-) using a B-Se bond to connect the fluorescent dye BODIPY and benzotrifluoride. The probe exhibited a highly selective fluorescence response to F- with a detection limit of 7.4×108 mol/L. The excitation and emission spectra of the probe in the NIR region avoid background fluorescence interference present in biological systems. The fluorescent imaging of HepG2 cells demonstrated that the newly developed probe should be broadly applicable to detect F- in living cells.In this study, we have designed and synthesized a new near-infrared （N1R） fluorescent probe （BODIPY-Se） to detect fluorine ions （F-） using a B-Se bond to connect the fluorescent dye BODIPY and benzotrifluoride. The probe exhibited a highly selective fluorescence response to F- with a detection limit of 7.4 × 10-8 mol/L. The excitation and emission spectra of the probe in the NIR region avoid background fluorescence interference present in biological systems. The fluorescent imaging of HepG2 cells demonstrated that the newly developed probe should be broadly applicable to detect F- in living cells.

关 键 词：HEPG2细胞 荧光探针 近红外 氟离子 细胞成像 应用 肾上腺素 荧光染料 

分 类 号：R346[医药卫生—基础医学]