A water-soluble fluorescence resonance energy transfer probe for hypochlorous acid and its application to cell imaging  

作　　者：JIA Jia MA HuiMin 

机构地区：[1]Beijing National Laboratory for Molecular Sciences, Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, ChineseAcademy of Sciences, Beijing 100190, China

出　　处：《Chinese Science Bulletin》2011年第31期3266-3272,共7页

基　　金：supported by the National Natural Science Foundation of China (20935005, 90813032, and 20875092);National Basic Research Program of China (2010CB933502, 2011CB935800);the Chinese Academy of Sciences (KJCX2-EW-N06-01)

摘　　要：A water-soluble fluorescence resonance energy transfer (FRET) probe for hypochlorous acid (HOCl), dansyl rhodamine B piperazinoacetohydrazide, was designed, synthesized and characterized. The dansyl moiety in the probe acted as a FRET donor and the rhodamine moiety acted as a FRET acceptor. The two moieties were connected by a HOCl-cleavable active bond, and cleavage of this linker decreased the FRET efficiency and increased the fluorescence intensity of the donor at 501 nm. The water solubility of the probe was improved compared with other probes by introduction of the cationic rhodamine fluorophore. As a result, the probe could be used to detect HOCl in aqueous biosystems with a linear range of 2-10 mol/L and a detection limit of 80 nmol/L (signal- to-noise = 3). The probe was successfully applied to fluorescence imaging of HOCl in HeLa cells.A water-soluble fluorescence resonance energy transfer （FRET） probe for hypochlorous acid （HOCl）, dansyl rhodamine B piperazinoacetohydrazide, was designed, synthesized and characterized. The dansyl moiety in the probe acted as a FRET donor and the rhodamine moiety acted as a FRET acceptor. The two moieties were connected by a HOCl-cleavable active bond, and cleavage of this linker decreased the FRET efficiency and increased the fluorescence intensity of the donor at 501 nm. The water solubility of the probe was improved compared with other probes by introduction of the cationic rhodamine fluorophore. As a result, the probe could be used to detect HOCl in aqueous biosystems with a linear range of 2-10μmol/L and a detection limit of 80 nmol/L （signal- to-noise = 3）. The probe was successfully applied to fluorescence imaging of HOCI in HeLa cells.

关 键 词：荧光共振能量转移 次氯酸 水溶性 细胞成像 应用 FRET 探针 罗丹明B 

分 类 号：Q2-33[生物学—细胞生物学]