A novel in vitro angiogenesis model based on a microfluidic device  

作　　者：DAI XiaoZhen CAI ShaoXi YE QunFang JIANG JiaHuan YAN XiaoQing XIONG Xin JIANG QiFeng WANG Albert Chih-Lueh TAN Yi 

机构地区：[1]Key Laboratory of Biorheological Science and Technology （Chongqing University） of Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China [2]Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China [3]Muscle and Motility Group, Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA [4]Chinese-American Research Institute for Diabetic Complications, Wenzhou Medical College, Chashan College Park, Wenzhou 325035, China [5]Pediatric Diabetes Research at KCHRI of the Department of Pediatrics, University of Louisville, Louisville, KY 40202, USA

出　　处：《Chinese Science Bulletin》2011年第31期3301-3309,共9页

基　　金：supported by the National Natural Science Foundation of China (10872224, 81000067, 30870607);the Fundamental Research Funds for the Central Universities (CDJXS10 23 11 15);CQNS grant (CSTC2008BB5192);Visiting Scholar Foundation of Key Laboratory of Biorheological Science and Technology (Chongqing University) of Ministry of Education

摘　　要：Angiogenesis is very important for many physiological and pathological processes. However, the molecular mechanisms of angiogenesis are unclear. To elucidate the molecular mechanisms of angiogenesis and to develop treatments for "angiogenesis-dependent" diseases, it is essential to establish a suitable in vitro angiogenesis model. In this study, we created a novel in vitro angiogenesis model based on a microfluidic device. Our model provides an in vivo-like microenvironment for endothelial cells (ECs) cultures and monitors the response of ECs to changes in their microenvironment in real time. To evaluate the potential of this microfluidic device for researching angiogenesis, the effects of pro-angiogenic factors on ECs proliferation, migration and tube-like structure formation were investigated. Our results showed the proliferation rate of ECs in 3D matrix was significantly promoted by the pro-angiogenic factors (with an increase of 59.12%). With the stimulation of pro-angiogenic factors gradients, ECs directionally migrated into the Matrigel from low concentrations to high concentrations and consequently formed multi-cell chords and tube-like structures. These results suggest that the device can provide a suitable platform for elucidating the mechanisms of angiogenesis and for screening pro-angiogenic or anti-angiogenic drugs for "angiogenesis-dependent" diseases.Angiogenesis is very important for many physiological and pathological processes. However, the molecular mechanisms of angiogenesis are unclear. To elucidate the molecular mechanisms of angiogenesis and to develop treatments for ＂angiogenesis-dependent＂ diseases, it is essential to establish a suitable in vitro angiogenesis model. In this study, we created a novel in vitro angiogenesis model based on a microfluidic device. Our model provides an in vivo-like microenvironment for endothelial cells （ECs） cultures and monitors the response of ECs to changes in their microenvironment in real time. To evaluate the potential of this microfluidic device for researching angiogenesis, the effects of pro-angiogenic factors on ECs proliferation, migration and tube-like structure formation were investigated. Our results showed the proliferation rate of ECs in 3D matrix was significantly promoted by the pro-angiogenic factors （with an increase of 59.12%）. With the stimulation of pro-angiogenic factors gradients, ECs directionally migrated into the Matrigel from low concentrations to high concentrations and consequently formed multi-cell chords and tube-like structures. These results suggest that the device can provide a suitable platform for elucidating the mechanisms of angiogenesis and for screening pro-angiogenic or anti-angiogenic drugs for ＂angiogenesis-dependent＂ diseases.

关 键 词：抗血管生成 微流体装置 模型基 体外 小说 内皮细胞增殖 分子机制 血管形成 

分 类 号：R318.0[医药卫生—生物医学工程]