Petal Development in Lotus japonicus  

作　　者：Lin Weng Zhaoxia Tian Xianzhong Feng Xin Li Shilei Xu Xiaohe Hu Da Luo Jun Yang 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200032, China [2]Key Laboratory of Plant Stress Research of Shandong Province, College of Life Sciences, Shandong Normal University, Jinan 250014 China [3]State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China

出　　处：《Journal of Integrative Plant Biology》2011年第10期770-782,共13页植物学报（英文版）

基　　金：supported by the Ministry of Agriculture of China for Transgenic Research(2011ZX08009003and2009ZX08009-112B);the National Natural Science Foundation of China(30930009and30528016);the Instituteof Plant Physiology and Ecology,SIBS

摘　　要：Previous studies have demonstrated that petal shape and size in legume flowers are determined by two separate mechanisms, dorsoventral （DV） and organ internal （IN） asymmetric mechanisms, respectively. However, little is known about the molecular mechanisms controlling petal development in legumes. To address this question, we investigated petal development along the floral DV axis in Lotus japonicus with respect to cell and developmental biology by comparing wild-type legumes to mutants. Based on morphological markers, the entire course of petal development, from initiation to maturity, was grouped to define 3 phases or 13 stages. In terms of epidermal micromorphology from adaxial surface, mature petals were divided into several distinct domains, and characteristic epidermal cells of each petal differentiated at stage 9, while epidermal cells of all domains were observed until stage 12. TCP and MIXTA-like genes were found to be differentially expressed in various domains of petals at stages 9 and 12. Our results suggest that DV and IN mechanisms interplay at different stages of petal development, and their interaction at the cellular and molecular level guides the elaboration of domains within petals to achieve their ideal shape, and further suggest that TCP genes determine petal identity along the DV axis by regulatincl MIXTA-like clene expression.Previous studies have demonstrated that petal shape and size in legume flowers are determined by two separate mechanisms, dorsoventral （DV） and organ internal （IN） asymmetric mechanisms, respectively. However, little is known about the molecular mechanisms controlling petal development in legumes. To address this question, we investigated petal development along the floral DV axis in Lotus japonicus with respect to cell and developmental biology by comparing wild-type legumes to mutants. Based on morphological markers, the entire course of petal development, from initiation to maturity, was grouped to define 3 phases or 13 stages. In terms of epidermal micromorphology from adaxial surface, mature petals were divided into several distinct domains, and characteristic epidermal cells of each petal differentiated at stage 9, while epidermal cells of all domains were observed until stage 12. TCP and MIXTA-like genes were found to be differentially expressed in various domains of petals at stages 9 and 12. Our results suggest that DV and IN mechanisms interplay at different stages of petal development, and their interaction at the cellular and molecular level guides the elaboration of domains within petals to achieve their ideal shape, and further suggest that TCP genes determine petal identity along the DV axis by regulatincl MIXTA-like clene expression.

关 键 词：CYC-like TCP genes epidermal cell Lotusjaponicus MIXTA-like genes petal. 

分 类 号：S685.12[农业科学—观赏园艺] TQ658[农业科学—园艺学]