超声波对实验性兔膝骨关节炎软骨细胞基质金属蛋白酶-1表达的影响  

The effect of ultrasonic therapy on expression of matrix-metalloproteinase-1 in cartilage cells of rabbit with knee osteoarthritis

作　　者：李卫平[1] 蹇睿[1] 胥方元[1] 

机构地区：[1]四川省泸州医学院附属医院康复科,泸州646000

出　　处：《中国康复医学杂志》2011年第10期911-914,共4页Chinese Journal of Rehabilitation Medicine

摘　　要：目的:探讨超声波治疗对兔膝骨关节炎(KOA)模型软骨细胞基质金属蛋白酶-1(MMP-1)表达的影响。方法:30只新西兰大白兔随机分为正常对照组、模型组和超声波治疗组,每组10只。将模型组和超声波治疗组的兔左膝于伸直位石膏固定6周制作KOA模型。造模后超声波治疗组实施超声波治疗2周。8周后取各组软骨观察膝软骨病理学形态改变、MMP-1表达的变化。结果:采用Mankin评分比较病理学形态改变,正常对照组与模型组、模型组与超声波治疗组间比较,差异均有显著性意义(P<0.01)。软骨免疫组化MMP-1测定,正常对照组与模型组、模型组与超声波治疗组间比较,差异均有显著性意义(P<0.01)。结论:超声波能有效治疗膝骨性关节炎,其作用机制可能与减少软骨细胞MMP-1的含量而利于软骨细胞的修复有关。Objective：To study the effect of ultrasound therapy on expression of matrix-metalloproteinase-1（MMP-1） in cartilage cells of rabbit with knee osteoarthritis（KOA）.Method：Thirty healthy New Zealand white rabbits were randomly divided into 3 groups：normal control group contained 10 rabbits,not modeling;the other 20 rabbits were randomly divided into model group and ultrasonic experimental group,being created osteoarthritis.The rabbits’ left knees were fixed within plaster cast in stretch position for 6 weeks to created KOA models.Then ultrasonic,experimental group was given ultrasonic treatment for 2 weeks.After 8 weeks cartilage changes on pathological morphology and expression of MMP-1 were observed in all groups.Result：By Mankin’s score the severity of pathological lesion in model group was higher than that in normal control group and ultrasonic experimental group（P〈0.01）.The expression of MMP-1 in model group was higher than that in normal control group and ultrasonic experimental group,the difference was significant（P〈0.01）.Conclusion：The ultrasonic treatment can effectively improve the rabbits’ KOA,and its mechanism probably is correlated with reducing MMP-1 level in cartilage cells,which will be helpful for repairing cartilage cells

关 键 词：骨关节炎 软骨细胞 超声波 基质金属蛋白酶-1 

分 类 号：R684.3[医药卫生—骨科学]