心肌缺血再灌注损伤模型大鼠心肌组织及血清肌钙蛋白Ⅰ表达和心肌肿瘤坏死因子α与重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白的干预  

Effects of recombinant human tumor necrosis factor receptor type Ⅱ antibody fusion protein on serum troponin Ⅰ and tumor necrosis factor alpha expression in myocardial tissue in a rat model of myocardial ischemia/reperfusion injury

作　　者：陈燕[1] 夏豪[1] 鲍翠玉[2] 陈红光[2] 汪新亮[2] 谢华强[1] 刘兰[1] 

机构地区：[1]武汉大学人民医院,湖北省武汉市436000 [2]咸宁学院心血管研究中心,湖北省咸宁市437100

出　　处：《中国组织工程研究与临床康复》2011年第37期6879-6882,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖北省自然基金(2008CDZ084);湖北省教育厅青年项目(Q20082801)~~

摘　　要：背景:心肌缺血再灌注时生成大量的肿瘤坏死因子α直接造成心肌的收缩功能下降。目的:观察药物重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白对大鼠缺血再灌注心肌损伤的影响。方法:成年雄性Wistar大鼠建立心肌缺血再灌注模型。药物干预组在再灌注前注射重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白,模型组给予生理盐水,并设立不造模的假手术组。再灌注后立即测量心肌梗死面积,ELISA检测再灌注后的心肌肿瘤坏死因子α及血清肌钙蛋白Ⅰ的含量,实时PCR检测心肌肿瘤坏死因子αmRNA的表达。结果与结论:与假手术组相比,模型组与药物干预组大鼠心肌肿瘤坏死因子α及其mRNA和肌钙蛋白的水平明显升高(P<0.05);与模型组相比,药物干预组心肌肿瘤坏死因子α及血清肌钙蛋白Ⅰ水平明显升高(P<0.05),心肌梗死体积与肿瘤坏死因子αmRNA的表达减少(P<0.05)。提示重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白能够减轻心肌缺血/再灌注损伤作用,改善大鼠的心功能。BACKGROUND：A lot of tumor necrosis factor α （TNF-α） generating during myocardial ischemia/reperfusion can cause worsened myocardial systolic function. OBJECTIVE： To investigate the effects of recombinant human tumor necrosis factor receptor typeⅡantibody fusion protein on myocardial tissue in a rat model of myocardial ischemia/reperfusion injury. METHODS：Myocardial ischemia/reperfusion injury was induced in adult male Wistar rats. In the drug intervention group, recombinant human tumor necrosis factor receptor typeⅡantibody fusion protein was injected before reperfusion. In the model group, normal saline was given before reperfusion. A sham-operated group with myocardial ischemia/reperfusion injury induction was set. Immediately after reperfusion, myocardial infarction area was measured, TNF-α and serum cardiac troponin Ⅰwere detected by ELⅠSA, and TNF-α mRNA expression was determined by real time-PCR. RESULTS AND CONCLUSION：Compared with sham-operated group, TNF-α, TNF-α mRNA, and serum troponinⅠ evels were significantly increased in the model and drug intervention groups （P 0.05）. Compared with model group, TNF-α and serum troponinⅠlevels, myocardial infarction area and TNF-αmRNA expression were significantly decreased （P 0.05）, in the drug intervention group. Results showed that recombinant human tumor necrosis factor receptor type Ⅱ antibody fusion protein can alleviate myocardial ischemia/reperfusion injury and improve cardiac function in rats.

关 键 词：心肌缺血再灌注损伤 肿瘤坏死因子Α 心肌肌钙蛋白Ⅰ 实时PCR 大鼠 

分 类 号：R318[医药卫生—生物医学工程]