不同免疫状态对肉仔鸡血液生化指标和细胞免疫应答的影响  

Effects of Different Immune Status on Blood Biochemical Parameters and Cellular Immune Response in Broiler Chickens

作　　者：冯焱[1,2] 杨小军[1] 王筱霏[1] 覃定奎[1] 尹瑞卿[1] 姚军虎[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]山西农业大学生命科学学院,山西太谷030801

出　　处：《中国农业科学》2011年第20期4309-4316,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31001017);陕西省科技攻关项目(2009K01-02);陕西省自然基金(2010JQ3002);陕西省饲料工程技术研究中心项目(2010ZDGC-02);动物营养学国家重点实验室项目(2004DA125184F0809)

摘　　要：【目的】研究不同免疫状态对肉仔鸡血液生化指标和细胞免疫应答的影响。【方法】选用健康情况良好1日龄AA肉公鸡180只,随机分为4组,每组5个重复,每重复9只。设定不同免疫应激模型,为无免疫组即对照组(no vaccine,NV),常规免疫组(conventional vaccine,CV),以及在常规免疫基础上注射细菌脂多糖的免疫亢进组(LPS)和环磷酰胺的免疫抑制组(CYP)。采用CD3/CD4/CD8三色流式细胞术、MTT法检测肉鸡免疫状态和非免疫状态下外周血中T淋巴细胞及其亚群的变化。【结果】与NV组相比,肉仔鸡的体增重和采食量均显著降低LPS组和CYP组(P<0.05),各处理料重比无显著差异(P>0.05),NV组与CV组生产性能指标无显著差异(P>0.05),各日龄处理间血清TP、GLU和CHLO含量均无显著差异(P>0.05)。第21和35天,LPS组和CYP组血清ALB含量显著低于CV组和NV组(P<0.05);第21天,LPS组血清α-AGP和BUN含量显著低于NV组(P<0.05),CPY和LPS均抑制T、B淋巴细胞增殖,LPS组CD4+/CD8+T细胞百分比显著低于NV组(P<0.05)。【结论】未进行免疫肉鸡可维持机体正常的生长性能和细胞免疫稳态,LPS和CYP的刺激可降低肉鸡采食量和体增重;随日龄增加肉鸡对CYP刺激的耐受性有所上升,LPS刺激可增强肉鸡细胞免疫应答反应。【Objective】 This experiment was conducted to determine the effects of different immune statuses on blood biochemical parameters and cellular immune response of broiler chickens.【Method】 One hundred and eighty 1-day-old AA broilers were allocated into four groups.Each treatment had 5 replicate cages of 9 broilers per replicate pen.This study will set different immune stress models,including the control group（no vaccines,NV）,conventional vaccines group（CV）,immune hyperthyroidism group（LPS） and immune suppression group（CYP）.The T lymphocytes and subgroups were isolated from periphery blood from immunized and unvaccinated birds and were detected by CD3/CD4/CD8 triple labelling by flow cytometric analysis and MTT assays,respectively.【Result】The results showed that different immunological statuses had no significant effect on feed conversion ratio of broiler chickens among 4 treatment（P0.05）.LPS group and CYP group had significantly lower broiler body weight gain and feed intake than NV group（P0.05）.Serum TP,GLU,and CHLO contents were no significantly different among treatments（P0.05）.On d 21 and 35,ALB of LPS and CYP groups was significantly lower than CV and NV groups（P0.05）,and ALB levels of NV and CV groups were not significantly different（P0.05）.On d 21,α-AGP of LPS group was significantly lower than CV group（P0.05）,BUN of LPS group was significantly higher than NV group（P0.05）.CPY and LPS inhibited the T,B lymphocyte proliferation,and CD4＋/CD8＋ T cell percentage of LPS group was significantly lower than NV group（P0.05）.【Conclusion】 Chickens with no vaccines maintained normal growth performance and immune response.LPS stress increased the cellular immune response of chickens.Tolerance of chicken to CYP increased with age.

关 键 词：AA肉鸡 免疫状态 血液指标 MTT法 淋巴细胞亚群 

分 类 号：S831[农业科学—畜牧学]