中国部分马铃薯产区马铃薯Y病毒(PVY)的株系分化与鉴定  

Study on the PVY strain differentiation and identification of some potato producing areas in China

作　　者：陈士华[1] 刘晓磊[1] 张晓婷[2] 吴兴泉[1] 

机构地区：[1]河南工业大学,河南郑州450001 [2]河南农业大学,河南郑州450002

出　　处：《河南农业大学学报》2011年第5期548-551,共4页Journal of Henan Agricultural University

基　　金：河南省教育厅自然科学基础研究项目(2006210003);河南工业大学校科研基金项目(07XJC008)

摘　　要：分别克隆了黑龙江、山西、贵州等3省内的4个马铃薯产区的PVY p1基因和cp基因,通过系统地基因序列分析对各分离物的株系种类进行了鉴定.结果表明中国马铃薯产区PVY株系分化现象明显,其中黑龙江省克山农场分离为PVYNTN株系;贵州省贵阳市分离物、威宁自治县分离物、山西省临县分离物为PVYN:O株系.PVYN:O株系分布普遍,可能已经成为中国部分马铃薯产区PVY的主要流行株系.The pl gene and cp gene of 4 PVY isolates from the Heilongjiang, Shanxi and Guizhou provinces were cloned. The strains of these PVY isolates were confirmed with the molecular identification method based on the sequence analysis of cp gene and pl gene. The results showed that the PVY strains in China differentiated obviously, the Keshan isolate from Heilongjiang province was PVYNTN strain, the Guiyang isolate, Weining isolate from Guizhou province and the Linxian isolate from Shanxi province were PVYN：O strain. As the PVYN：O strain,it was widespread in some areas has probably become the main epidemic strain in China.

关 键 词：马铃薯Y病毒 株系 分子鉴定 

分 类 号：S432.1[农业科学—植物病理学]