猪细小病毒感染Marc-145细胞后对其分泌白细胞介素18转录时相的影响  

Study on influence of transcriptional profiles of interleukin-18 in Marc-145 cell cultures following infection with porcine parvovirus

作　　者：党玉丽[1] 哈斯通拉嘎[2] 耿静微[3] 王瑞宁[3] 郭东辉[3] 胡清林[3] 魏战勇[3] 

机构地区：[1]河南农业大学理学院,河南郑州450002 [2]郑州牧业工程高等专科学校,河南郑州450011 [3]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业大学学报》2011年第5期568-570,584,共4页Journal of Henan Agricultural University

基　　金：河南省重点科技攻关项目(08210213007)

摘　　要：为了解猪细小病毒(PPV)感染Marc-145细胞后引起细胞炎性反应特别是白细胞介素18(IL-18)的反应,探讨宿主-病毒之间的作用关系,运用荧光定量PCR技术,测定和分析了PPV感染Marc-145细胞后引起的病毒DNA含量的变化和IL-18的分泌水平.结果表明,IL-18的表达量在1 h无明显变化、在3,24 h增加,48 h轻微下调.表明猪细小病毒感染可引起Marc-145细胞分泌IL-18增加.In order to survey the host inflammatory responses, in particular interleukin-18 responses of Marc-145 infected with porcine parvovirus （PPV） , and to study the host-PPV interaction, PPV was to used infect the Marc-145 cells to measure and analyze the viral DNA using real-time PCR. The transcript level of interleukin-18 was detected by real-time PCR too. The results showed that the transcript level of interleukin-18 had no obvious change 1 h after inoculation, increased 3 and 24 h after inoculation, and was slightly lower than that of the control 48 h after inoculation. The results indicate infection PPV can upregulate the secretion of interleukin-18 in Marc-145 cell.

关 键 词：猪细小病毒 MARC-145细胞 白细胞介素18 转录时相 

分 类 号：S858.28[农业科学—临床兽医学]