NF-κB在砷诱导人正常膀胱上皮细胞COX-2表达中的作用  

Effects of NF-κB on COX-2 expression in arsenite exposed SV-HUC-1 cells

作　　者：席淑华[1] 王惠惠[1] 王菲[1] 刘盛男[1] 孙贵范[1] 

机构地区：[1]中国医科大学公共卫生学院地球化学性疾病研究室辽宁省砷生物学作用与砷中毒重点实验室,辽宁沈阳110001

出　　处：《中国工业医学杂志》2011年第5期327-329,356,共4页Chinese Journal of Industrial Medicine

基　　金：国家自然科学基金项目(81072244);辽宁省教育厅科学技术研究项目(2009A756):砷致膀胱癌机制研究

摘　　要：目的观察NF-κB核转录因子在砷诱导人正常膀胱上皮细胞(SV-HUC-1)环氧化酶-2(COX-2)表达中的作用。方法在细胞培养液中加入不同浓度的NaAsO2,24 h后提取细胞总RNA和核蛋白,用RT-PCR和Westernblot方法分别分析COX-2 mRNA表达和NF-κB蛋白水平;选择COX-2高表达的NaAsO2处理组,再用NF-κB抑制剂(PDTC)处理,观察COX-2 mRNA表达水平的变化。结果 4、8μmol/L NaAsO2处理细胞后,COX-2 mRNA表达水平显著高于对照组(P<0.05);4μmol/L NaAsO2处理组细胞NF-κB蛋白水平也显著提高,高于对照组,而10μmol/LNaAsO2处理组细胞NF-κB蛋白水平低于对照组(P<0.05);同时用4μmol/L NaAsO2和PDTC共同处理细胞后,COX-2 mRNA表达水平降低,显著低于单纯用4μoml/L NaAsO2处理细胞的COX-2 mRNA表达水平(P<0.05)。结论低剂量的砷能够诱导人正常膀胱上皮细胞COX-2 mRNA和NF-κB蛋白表达水平的增高,砷诱导的核转录因子NF-κB活化在砷诱导的COX-2表达水平增高中起一定作用。Objective To explore the effects of NF-κB on COX-2 mRNA expression in SV-HUC-1 cells exposed to arsenite.Methods Cells were cultured in medium contained 0,1,2,4,8 and 10 μmol/L arsenite or PDTC,the inhibitor of NF-κB for 24 h,then the expressions of COX-2 mRNA and nuclear protein NF-κB were determined by RT-PCR and Western blot,respectively.Results Compared with those in control group,the expressions of COX-2 mRNA in cells exposed to 4 and 8 μmol/L arsenite and the expressions of NF-κB in cells exposed to 4 μmol/L arsenite were increased significantly（P〈0.05）,while expressions of NF-κB nuclear proteinum in the cells exposed to 10 μmol/L arsenite were decreased significantly.On the other hand,expressions of COX-2 mRNA in cells exposed to both 4 μmol/L arsenite and PDTC were lower significantly than those in the cells only exposed to 4 μmol/L arsenite（P〈0.05）.Conclusion Findings from this study suggested that low dose of arsenic could induce the expression of COX-2 mRNA and NF-κB in SV-HUC-1 cells,while the activation of NF-κB induced by arsenic might play some role in the process mentioned above.

关 键 词：砷 人膀胱上皮永生化细胞(SV-HUC-1) 环氧化酶-2(COX-2) 核转录因子NF-ΚB 

分 类 号：R99[医药卫生—毒理学]