重组鲨肝刺激物质类似物对CCl_4诱导的急性肝损伤的保护作用  

Protective Effect of r-sHSA on Acute Hepatic Injury Induced by CCl_4 in Mice

作　　者：杭永付[1] 张晓园[2] 薛晓燕[1] 王颖[2] 叶波平[2] 丁选胜[1] 

机构地区：[1]中国药科大学临床药学教研室,江苏南京210009 [2]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《药物生物技术》2011年第5期438-440,共3页Pharmaceutical Biotechnology

基　　金：国家高技术研究发展计划(863计划)重点项目(2007AA091402)

摘　　要：探讨重组鲨肝刺激物质类似物(r-sHSA)对CCl_4急性肝损伤模型的保护作用。采用CCl_4花生油溶液(0.15%,ip)诱导小鼠急性肝损伤,用改良赖氏法测定血清ALT、AST、MDA水平,并观察病理变化。结果显示r-sHSA能显著降低CCl_4所致小鼠ALT和AST升高,恢复AST/ALT比值,降低MDA的含量。病理切片显示r-sHSA能明显减轻CCl_4对肝组织的损伤。r-HSA对CCl_4急性肝损伤小鼠具有一定的保护作用。To investigate the protective effects of recombinant shark hepatical stimulator analogue （r-sHSA） on acute hepatic injury induced by carbon tetrachloride （ CCl4 ） in mice, the acute liver-injury model of ICR mice induced by CCl4 was established by a single intraperitoneal injection of CCl4 （0.15%） at a dose of 20 mg/kg body weight. Serum ALT and AST activities were analyzed. Histopathological changes in the liver were also examined. The results showed r-sHSA could significantly decrease the activities of mouse serum AI,T and AST caused by CC14,recuperate AST/ALT ratio and reduce the content of MDA. Hepatic histological examination showed that r-sHSA could reduce the injury to liver tissue, r-sHSA has a protective effect on acute liver injury by CCl4 in mice.

关 键 词：鲨肝刺激物质类似物(r—sHSA) CCL4 保护作用 

分 类 号：R963[医药卫生—微生物与生化药学]