8份不结球芸薹属蔬菜种质的POD、SOD和EST同工酶比较分析  

Comparative Study on Isoenzymes of Peroxidease,Superoxide Dismutase and Esterase in Eight no-heading Brassica Vegetables Germplasms

作　　者：舒英杰[1] 周玉丽[1] 赵晓雪[1] 邓倩妹[1] 

机构地区：[1]安徽科技学院,安徽凤阳233100

出　　处：《种子》2011年第10期25-27,32,共4页Seed

基　　金：安徽省高校青年教师资助项目(2006jql194)

摘　　要：采用垂直平板聚丙烯酰胺凝胶电泳技术,对8份不结球芸薹属蔬菜种质的过氧化物酶(POD)、超氧化物歧化酶(SOD)和酯酶(EST)3种同工酶进行了分析研究,并利用聚类分析的方法探讨其亲缘关系。结果表明,8份不结球芸薹属蔬菜种质的POD同工酶分离出3条酶带,其中p3为8份种质所共有酶带,p2为2份芥菜种质特有酶带;SOD同工酶共分离出4条酶带,其中s1、s2和s3为共有酶带,s 4为2份芥菜种质特有酶带;EST同工酶共分离出5条酶带,其中e 1、e 2、e 4和e 5为共有酶带,2份芥菜种质缺失e 3酶带。在遗传距离0.66处可将8份不结球芸薹属蔬菜种质分为芸薹种蔬菜和芥菜种蔬菜两大类。Isoenzymes of Peroxidease （ POD ）, Superoxide dismutase （ SOD ） and Esterase （EST） in eight noheading brassica vegetables germplasms were studied by polyacrylamide gel electrophoresis （PAGE）. The species relation of eight no-heading showed that 3 peroxidases,4 supe brassica vegetables germplasms were analyzed by clustering. The results roxide dismutase and 5 esterase were separated, p 2 of peroxidases and s 4 of superoxide dismutase were the particular enzyme bands in Brassica juncea, other enzyme bands were common in Brassica campestris and Brassica juncea. Brassica campestris and Brassica juncea of eight no-heading brassica vegetables germplasms could be separated when genetic distance was 0.66, each group had obvious different enzyme bands of POD ,SOD and EST.

关 键 词：不结球芸薹属蔬菜 POD同工酶 SOD同工酶 EST同工酶 聚类分析 

分 类 号：S634.4[农业科学—蔬菜学]