西方马脑炎病毒抗体快速检测试纸条的研制  

Design of a test strip for rapid detection of antibodies against Western equine encephalitis virus

作　　者：王林林[1,2] 杨宇[1] 王旺[1] 王振东[2] 王静[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123 [2]沈阳农业大学生物科学技术学院,沈阳110866

出　　处：《中国媒介生物学及控制杂志》2011年第5期421-423,共3页Chinese Journal of Vector Biology and Control

基　　金：质检公益项目(2007GYJ024;2007GYJ023);国家质检总局科技计划项目(2010IK211)~~

摘　　要：目的研制一种快速检测西方马脑炎病毒(WEEV)抗体的试纸条。方法利用胶体金标记和免疫层析技术,用25 nm胶体金标记葡萄球菌A蛋白,并用硝酸纤维膜包被WEEV抗原及羊抗兔IgG作为检测带和质控带,研制出WEEV抗体快速检测试纸条。对该试纸条的敏感性、特异性和稳定性做出评价,并拟合检测曲线进行半定量检测。在健康人血清、兔血清和鼠血清中添加WEEV抗体模拟污染样品,评价该方法的检测能力。结果该试纸条可在20 min内完成定性和半定量检测,灵敏度为120 ng/ml,模拟样品的检测灵敏度也相同。线性范围120～1200 ng/ml。对发病症状相似以及近缘的其他病毒进行检测,均无非特异反应。试纸条在37℃下保存1个月,检测结果不变。结论该试纸条具有快速、简便、灵敏、特异、稳定的特点,适用于现场检测。Objective To develop a test strip for rapid detection of antibodies against Western equine encephalitis virus（WEEV）.Methods Staphylococcal protein A was labeled with 25 nm colloidal gold via immune-chromatography;cellulose nitrate-coated WEEV antigens and goat anti-rabbit antibodies were used as the test zone and the control zone,respectively.The sensitivity,specificity and stability of the test strip were evaluated and semi-quantitative detection was conducted with a curve of best fit.The detection capability of the test strip was assessed using healthy human serum,rabbit serum and rat serum samples containing simulated WEEV antibodies.Results The qualitative and semi-quantitative detection was completed within 20 min,with a resulting sensitivity of 120 ng/ml in simulated samples.The linear range of sensitivity was 120-1200 ng/ml.No nonspecific reaction was observed when applied to other homologous viruses and those leading to similar symptoms.Storage at 37 ℃ over a month did not make a difference in the test result.Conclusion The test strip is a fast,simple,sensitive,specific and reliable tool for detection of WEEV and is suitable for field application.

关 键 词：西方马脑炎病毒 免疫层析技术 胶体金试纸 定性检测 定量检测 

分 类 号：R373[医药卫生—病原生物学]