羧酸酯酶用于家蝇高效氟氯氰菊酯抗性监测的研究  

Application of carboxylesterase in beta-cyfluthrin resistance surveillance of Musca domestica

作　　者：陶卉英[1] 马红梅[1] 柳小青[1] 李卫民[1] 郭学俭[1] 

机构地区：[1]南昌市疾病预防控制中心消毒与病媒生物防制科,江西南昌330038

出　　处：《中国媒介生物学及控制杂志》2011年第5期453-455,458,共4页Chinese Journal of Vector Biology and Control

摘　　要：目的探讨羧酸酯酶(CarE)与家蝇高效氟氯氰菊酯抗性之间的关系,并将CarE应用于不同生境家蝇高效氟氯氰菊酯抗性的早期检测。方法采用微量点滴法进行生物测定;采用Asperen方法进行CarE活性测定。结果生物测定结果表明,除垃圾中转站家蝇种群外,居民区、餐饮店和农贸市场家蝇种群对高效氟氯氰菊酯产生了不同程度的耐药性或抗药性,抗性指数依次为1.84、15.31、3.19、8.84倍。CarE活性测定结果表明,除垃圾中转站种群外,其它3个家蝇野外种群与敏感品系相比显著升高(P<0.05),而且家蝇CarE的比活力与其对高效氟氯氰菊酯的抗性之间呈显著的正相关。对不同生境家蝇种群CarE个体频率分布情况的研究也发现,农贸市场、餐饮店和居民区家蝇种群与敏感品系间有明显的重叠现象。此外,3个家蝇种群高活性的个体频率分别为居民区种群75%,农贸市场种群72%,餐饮店种群54%。结论 CarE活性的升高与家蝇高效氟氯氰菊酯的抗性有关;不同个体CarE活性分布情况表明农贸市场、餐饮店和居民区家蝇种群基本上是抗性杂合种群,与生物测定结果基本相符。此外,根据不同生境家蝇种群中高活性个体频率的发展趋势,建议居民区在家蝇防制时应限制使用高效氟氯氰菊酯,换用或轮用其他杀虫剂,以避免或延缓抗性的发展;其他生境应慎用高效氟氯氰菊酯。Objective To determine the association between carboxylesterase（CarE） and beta-cyfluthrin resistance in Musca domestica in various habitats,and monitor this resistance at an early phase by using carboxylesterase.Methods Biological assessment was conducted using dripping titration and the activity of carboxylesterase was determined by the Asperen method.Results Except for the population in transfer stations,the three populations of M.domestica in residential areas,restaurants and farmer＇s market presented different resistance levels to beta-cyfluthrin with resistance ratios of 1.84,15.31,3.19 and 8.84,respectively.The CarE activities in the three populations were significantly elevated（P0.05）,while 75%,54% and 72% of them showed remarkably high activities,respectively.Overlapping susceptible strains were found in the three populations except for that in transfer stations.Conclusion Increased CarE activitiy was associated with beta-cyfluthrin resistance in M.domestica.The distribution of high CarE-activity strains showed that the populations in those areas were hybrid resistant strains,which was consistent with the bioassay results.Based on this finding,it is advisable to restrict the use of beta-cyfluthrin in residential areas and use other pesticides as an alternative to avoid or defer the occurrence of resistance and apply this agent with caution in other habitats.

关 键 词：家蝇 高效氟氯氰菊酯 羧酸酯酶 抗药性 

分 类 号：R384.2[医药卫生—医学寄生虫学] S481.4[医药卫生—基础医学]