斑蝥在生物转化前后斑蝥素含量比较  

Study on the Change of the Content of Cantharidin in Mylabris befere and after Biortransfermation

作　　者：许金国[1] 张建辉[1] 陈建伟[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210000

出　　处：《中药材》2011年第8期1180-1182,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:利用斑蝥与红栓菌进行生物发酵转化的新方法,探索提高中药斑蝥中可利用的斑蝥素含量,增加斑蝥素的溶出率,从而扩大斑蝥资源的更有效利用。方法:以斑蝥素为标准品,用气相色谱法测定斑蝥中生物转化前后斑蝥素含量。采用HP-5气相色谱柱,香兰素为内标,柱温为175℃保持5 min,4℃/min升到240℃,保持5min,建立了斑蝥素含量测定方法。结果:建立的测定方法具有良好的重现性,转化前斑蝥中可溶出斑蝥素的含量为0.70%,转化后可溶出斑蝥素的含量为1.29%,转化前后测得斑蝥素的含量有明显提高。结论:利用红栓菌发酵对斑蝥素含量的提高具有重要的意义,为斑蝥资源的有效利用提供实验依据。Objective：To compare the change of the content of cantharidin in Mylabris before and after biotransformation.Methods： The content of cantharidin was determined by gas chromatography（GC）.The conditions of GC were as follows： HP-5 column,vanillin as internal standard,the column temperature maintained at 175℃ for 5 min,rised to 240℃ at the speed of 4℃/min and the remained for 5 min.Results：The GC method was good reproducibility.The content of cantharidin in Myabris before and after botransformation was 0.7% and 1.29% respectively.After biotranformation,the content of cantharidin was increased.Conclusion： Mylabris fermentated with Trametes cinnabarina has great significance for increasing the content of cantharidn and provides experimentd basis for efficient use of Mylabris.

关 键 词：气相色谱法 斑蝥 斑蝥素 红栓菌 生物发酵与转化 含量 

分 类 号：R282.74[医药卫生—中药学]